Bridging Flow Cytometry with New Technologies

Bridging Flow Cytometry with New Technologies

Recorded On: 12/16/2015

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About the Presenters

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Monica DeLay
Manager Flow Cytometry Core
Cincinnati Children's Hospital

After receiving an MS in biology, Monica DeLay performed research for over a decade in the area of autoimmune diseases, focusing on the structure and function of human major histocompatibility complex (MHC) and its role in modulating disease. In 2008 she began managing the Research Flow Cytometry Core at Cincinnati Children’s Hospital in Cincinnati, Ohio. Over the last nine years, she has helped facilitate the growth of the core in instrumentation, infrastructure, staff, and clientele. She is co-founder and president of the Ohio River Valley Cytometry Association, an organization connecting advancements in cytometry technology to local scientists. She is the former chair of the ISAC SRL Services Committee and former co-chair of the SRL Emerging Leaders Committee. She is a member and past co-chair of the ABRF Flow Cytometry Research Group whose mission is to understand alterations in cell function and develop best practices associated with cell sorting. She is a member of the organizing committee for the Core Managers Workshop for the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA), and she has served as a Council member for ISAC.

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Sherry Thorton, PhD

Dr. Sherry Thornton received her PhD in developmental biology from the University of Cincinnati College of Medicine. Her postdoctoral fellowship involved the use of flow cytometry in antigen-specific responses in the laboratory of Dr. Raphael Hirsch. She then joined the faculty in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center (CCHMC) and became flow core director for the Rheumatology Center Grant (NIH P30 AR47363). She has been extensively involved with the correlation of gene expression profiles with cell types (determined by flow cytometry analysis) of PBMC from large cohorts of Juvenile Idiopathic Arthritis patients (>500) and control specimens. Her research interests also include examination of cell types involved in the pathogenesis of arthritis. In June of 2008, Dr. Thornton led the consolidation of flow cytometry at CCHMC, and she became Director of the Research Flow Cytometry Core that houses all flow cytometry instrumentation involved in basic research at CCHMC. Dr. Thornton is active locally, nationally, and internationally in the flow cytometry community. She is an active member of the International Society for Advancement of Cytometry, a founding member of the Ohio River Valley Cytometry Association, steering committee member of the Great Lakes International Imaging and Flow Cytometry Association, and a member of the Career Development Committee of the Association of Biomolecular Resource Facilities.

Webinar Summary

Novel applications, such as single-cell RNASeq, induced pluripotent stem cell (iPSC) generation, and CRISPR-mediated genome editing, have revolutionized the way that cells can be analyzed and manipulated to investigate developmental pathways and disease processes. These technologies can also benefit from using flow cytometry as an upstream or downstream process and, when combined, may require the involvement of several Shared Resource Labs (SRLs) from sample collection to data analysis. This webinar will highlight research projects that have utilized the above applications in combination with flow cytometry and/or cell sorting, discuss critical parameters that should be considered for successful experimental outcomes and provide strategies for coordination among core facility staff and investigators. This discussion is aimed to provide ideas on the integration of new technologies from other cores into investigator-driven projects that utilize their SRL.

CMLE Credit: 1.0

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Bridging Flow Cytometry with New Technologies
Recorded 12/16/2015
Recorded 12/16/2015 CYTO U Webinar presented by Monica DeLay, MS & Sherry Thornton, PhD
Handout - Bridging Flow Cytometry with New Technologies by Monica DeLay and Sherry Thornton
Open to download resource.
Open to download resource.
CMLE Evaluation Form
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11 Questions CMLE Evaluation Form
Completion Credit
1.00 CMLE credit  |  Certificate available
1.00 CMLE credit  |  Certificate available