A CYTO U Webinar presented by Jane Srivastava
Keywords: Equity, Training, Inclusivity
About the Speaker
Jane Srivastava, Core Director for Flow Cytometry, Light Microscopy and Histology, J. David Gladstone Institutes
Originally from Christchurch, New Zealand, Jane Srivastava (she/her) completed her undergraduate studies in plant and microbial biology at the University of Canterbury. She worked as a lab technician before discovering flow cytometry in 2003 and has been in the industry ever since, working as a flow lab manager at Imperial College London and designing practical and theoretical flow courses for undergraduate and postgraduate students. Jane is now director of the Flow Cytometry Core at the Gladstone Institutes and has been a mentor for Gladstone’s PUMAS (Promoting Underrepresented Minority Advancement in the Sciences) summer internship program. In 2024, Jane took over the directorship of the Light Microscopy and Histology at Gladstone Institutes. Jane considers herself a career technician and is passionate about bringing recognition and support to those working in a scientific technical career
Webinar Summary
Although education methodology has advanced considerably form even a few years ago, there is still a general need to include equitable teaching practices in training sessions offered by shared resource facilities. Jane will summarize and give examples of these practices, from how to introduce feedback literacy for both students and staff, to how to utilize technological platforms to enhance student learning.
Learning Objectives
Learn how to modify training to make it more inclusive. Understand how equitable training practices lead to less repetition of key concepts and reduces staff burden
Who Should Attend
Anyone that is offering training to people using an SRL.
A CYTO U Webinar presented by Adrian Liston
Keywords: FlowCodes, in vivo analysis, T regulatory cells
About the Speaker
Adrian Liston, PhD - Professor of Pathology, University of Cambridge
Dr. Liston has extensively worked in the cellular control over immune/tolerance switches, and how molecular defects in these switches can lead to pathologies ranging from autoimmunity and primary immunology to diabetes and neuropathology. By researching a broad range of pathologies, and using both patient samples and animal models, Liston’s research is able to identifying the common cellular pathways to pathology. Liston has been awarded the Francqui Chair, the Eppendorf Prize and fellowship to the Academy of Medical Sciences and the Royal Society of Biology. Liston currently serves as the Editor-in-Chief of Immunology & Cell Biology.
Webinar Summary
The tissues are the site of many of the most important immunological reactions, yet the immunology of the tissues has remained relatively opaque. Recent studies have identified Foxp3+ regulatory T cells(Tregs) in several non-lymphoid tissues. These tissue-resident populations have been ascribed unique characteristics based on comparisons to lymphoid Tregs. To understand key aspects of this tissue Treg population, we need technological approaches thatare suitable for the assessment of low cell number sources with high dimensionality, such as flow cytometry, to be coupled with massively parallel screening technologies (e.g. CRISPR screens, TCR clonality screens). We modified the ProCodes technology into FlowCodes, a flow cytometry-based platform suitable for in vivo analysis. Using this system, we found that T cell receptors (TCRs) extracted from tissue Tregs conferred Treg fate and also multi-organ homing. These results demonstrate that tissue-resident Tregs are largely constituted by broadly self-reactive Tregs, characterized by transient multi-tissue migration and a common residency program.
Learning Objectives
Regulation of T cell mediated tolerance Understanding tissue resident versus lymphoid origin regulatory T cells Development and Utilization of FlowCodes: A flow cytometry-based platform suitable for in vivo analysis
Who Should Attend?
General interest to beginner and advanced flow cytometrists SRL staff interested in development and implementation of multiplexing technology Immunologists interested in T cell biology
A CYTO U Webinar presented by Florian Mair
Keywords: Education, Panel Design, 50-Color, Training
About the Speakers
Florian Mair - Scientific Director of Flow Cytometry Core Facility, ETH Zurich, Switzerland
Florian is currently working as the Scientific Director of the Flow Cytometry Core Facility and Senior Scientist Immunology at ETH Zurich, Switzerland. After graduating with an MSc in Molecular Biology from the University of Vienna, he received his PhD in Molecular Life Sciences from the University of Zurich in 2014, and spent several years at the Fred Hutchinson Cancer Center in Seattle, USA. During the past decade, he has been involved extensively with different high-dimensional cytometry platforms as well as scRNA-sequencing techniques, including multi-omic methods. His main scientific interests are the function of dendritic cells and T cells in non-lymphoid tissues during health and disease, teaching best practices in cytometry and developing new flow cytometry panels and approaches.
Webinar Summary
To understand the function of the human immune system it is imperative to capture as much information as possible from often size-limited human samples. To do so, we have developed the first 50-color spectral flow cytometry panel to comprehensively study the functional state of the human immune system in PBMCs and tissue samples. The panel contains lineage markers for all major immune cell subsets, and an extensive set of phenotyping markers focused on the activation and differentiation status of the T cell and dendritic cell (DC) compartment. The panel has also been tested to be suitable for cell sorting, which allows very fine-grained isolation of immune subsets utilizing 50 markers at the same time.
To establish such a complex panel, we utilized a new metric termed unmixing spreading error, that evaluates the fluorochrome-specific increase in background that is inherently generated when unmixing highly complex panels. In this presentation we show the systematic workflow we used to develop the panel, how we evaluated panel performance, and how OMIP-102 was developed to its final state.
A CYTO U Webinar presented by Mariela Bollati-Fogolin and Ziv Porat
Keywords: SRL – End Users – Training
the Speakers
Ziv Porat - Head of Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science, Israel
Ziv Porat obtained his PhD and post doctorate studies in Cell Biology from the Weizmann Institute of Science, Israel. Since 2011 he is part of the Weizmann Institute Flow Cytometry unit, and serves as head of the unit since 2018 as a Senior Staff Scientist. He specializes in Imaging Flow Cytometry, and participated in more than 500 different projects utilizing this technology. He took part in more than 100 publications in this field, including development of novel Imaging Flow Cytometry applications. He participates and lectures in numerous international conferences, including the CYTO meetings since 2015, and he is part of the ISAC emerging leaders program (2019-2024). Dr. Porat is the organizer of the Israeli annual Imaging Flow Cytometry meetings since 2012. He is the co-founder of the Flow Cytometry Society of Israel (FCSI) and organizer of the Israeli flow cytometry conferences and workshops.
Mariela Bollati - Head Cell Biology Unit, Institut Pasteur de Montevideo, Uruguay
Since 2006, Dr Mariela Bollati-Fogolín has been working at the Institut Pasteur de Montevideo, Uruguay, as Head of the Cell Biology Unit (CBU). There, she has been instrumental in establishing a vibrant and internationally recognized flow cytometry and cell culture facility, one of the first of its kind in South America. Managing the CBU Shared Resource Laboratory and its staff, she provides support and assistance to research scientists and companies throughout the Latin American region. She has actively participated in organizing several hands-on flow cytometry workshops. She has served on the Council (2020-2023) of the Uruguayan-Argentine Flow Cytometry Society (GRCF). At present, she is the ISAC CYTO Women Taskforce chair and serves as the Uruguayan Deputy Director of the Latin American Center for Biotechnology
Webinar Summary
Drs. Mariela Bollati-Fogolín and Ziv Porat will co-present the upcoming webinar about developing and delivering an effective training program for Shared Resource Laboratories. This webinar will focus on how to teach users how to utilize flow cytometry equipment properly. By examining real-world case studies, the presenters will illustrate the potential pitfalls of allowing inexperienced individuals unsupervised access to cytometry equipment. Additionally, they will emphasize the critical role of a quality management system (QMS) in ensuring the success of any internal training program.
This webinar is the second installment in a three-part series dedicated to training resources. Last month's webinar, presented by Kathy Daniels and Derek Davies, explored external training solutions. The series will conclude in December with a presentation by Jane Srivastava on leveraging modern educational theories to create equitable training programs.
Learning Objectives
In this webinar the attendees will learn: 1- Two case studies to deliver an Internal Training Program 2- The importance of a good training and education for end users 3- The relevance of having a quality management system (QMS) for Internal Training Program
Who Should Attend
SRL managers, staff and users of shared resource laboratories. Users of flow cytometry technologies or anyone who is interested in flow cytometry education.
Kathy currently works to provide support on flow cytometry experimental design and oversight for global clinical trials. She also serves on the Steering Committee for the MetroFlow regional cytometry group and volunteers within multiple committees for the International Society for the Advancement of Cytometry. In the past she has worked in both pharmaceutical and academic settings in the capacity of a flow cytometry shared resource facility where she supported end users in experimental design, education, experimental education and interpretation of data. Prior employees include The Whitehead Institute for Biomedical Research, Memorial Sloan Kettering Cancer Center and Regeneron Pharmaceuticals.
Derek Davies - Cytometry Trainer, Educator and Consultant, Derek Davies Cytometry Derek has been involved in Cytometry for over 40 years. He first used cytometry to measure DNA content in cervical cells but quickly realised the power of the technology. For 25 years he ran a Flow Cytometry SRL at the London Research Institute and then the Francis Crick Institute in London, UK. In that time he trained thousands of users and also developed the careers of SRL staff. In 2018, he became the Science Technology Platform Training Lead at the Francis Crick Institute where he developed training for users external to the Institute. In 2023, he transitioned to semi-retirement but is still active in education, training and consultancy. He is current Chair of flowcytometryUK, sits on the Cytometry Committee of the Royal Microscopical Society and is a former ISAC Councilor.
Webinar Summary • Introductions • Start w/ importance of training - rigor and reproducibility • Responsibilities of the SRL in training and education • Bottlenecks and challenges faced when handling training internally at an SRL • What are the outsourcing options? • Theory, practical, in person, virtual, core curriculum • Vendor based training (on site vs virtual) • Free & Paid Training Resources (benefits vs limitations) • Annual Training Courses Examples • How to evaluate these training courses? • Metrices for evaluation • How can this be done before you commit to one (or more) solutions?
Learning Objectives In this webinar we hope to have everyone leave with a better understanding of why training and education is so critical and how to evaluate training opportunities that are available outside the SRL. As part of this, we will share what some options are, and how to evaluate them and their effectiveness.
Who Should Attend
SRL managers, staff and users of shared resource laboratories. Users of flow cytometry technologies or anyone who is interested in flow cytometry education
Why can't they play nicely? Navigating unexpected challenges in designing new flow cytometry panels and expanding existing ones - Anna C. Belkina, MD, PhD & Kelly Lundsten
Think Like an Engineer – Early Detection and Troubleshooting of Cytometer Problems - Rachael Sheridan, PhD SCYM (ASCP)CM & Matthew Goff
A Beginner's Guide to Computational Cytometry: From Algorithms to Applications - Sofie Van Gassen
Label-Free Imaging and High Dimensional Analysis - Peter O'Toole, PhD & Karen Hogg, PhD
Assay Design and Standardization: What Can Researchers Learn From Clinical and Vice Versa? - Lyana Setiawan, MD, PhD, Paul Hutchinson, Paresh Jain
Image Cytometry Analysis Using AI Techniques - Aida Meghraoui, PhD, PharmD & Polat Goktas, PhD
A CYTO U Webinar presented by David Leclerc
Keywords: SRL Promotion, Marketing Approaches, Communication with Users, Optimizing the Value of SRL Services
About the Speaker
David Leclerc
Director, CAT Facility at the University of Chicago
David Leclerc is the Director of the Cytometry and Antibody Technology (CAT) Facility at the University of Chicago. With over 20 years of expertise in the field, David is passionate about implementing cutting-edge technologies and fostering innovative multi-media solutions to encourage its adoption within the research community. Beyond his role at the University of Chicago, David is a tenured member of the GLIIFCA Board of Directors, President of the Chicago User Group, Creator of the FixYourFlowDepot (a video repository on cytometry maintenance and repair), and host of the ChUG Podcast.
Webinar Summary
An important function of a shared service laboratory (SRL) is the promotion of novel technologies for the benefit of the research community. This webinar introduces a systematic method to help SRL’s optimize their efforts when advertising new instruments/services within their facility. The GSTIC approach (Goal/Strategy/Tactics/Implementation/Control) allows SRLs to design a methodical approach to identify objectives/tactics, and most importantly, a clear set of expectations to evaluate the success of your strategies.
Learning Objectives
1. Understanding the GSTIC Approach: Participants will gain knowledge about the GSTIC approach and its application in increasing the value of services within a Shared Service Laboratory (SRL).
2. Applying GSTIC for Marketing Goals: Participants will learn how different components of the daily activities within an SRL can be utilized to support marketing objectives, emphasizing practical application.
3. Exploring Communication Channels: Participants will gain insights into the various communication channels that exist between an SRL and its users. This includes an examination of the pros and cons associated with different options, fostering a comprehensive understanding of effective communication strategies within an SRL context
A CYTO U Webinar presented by Caroline E. Roe, MLI
Keywords: High dimensional cytometry, Spectral flow, Mass cytometry
About the Speaker
Caroline E. Roe, MLI Managing Director, Cancer and Immunology Core Vanderbilt University, Nashville, TN, USA
Ms. Roe received her B.S. in Genetics, Cell Biology, and Development from the University of Minnesota-Twin Cities and Master of Laboratory Investigation from Vanderbilt University in Nashville, Tennessee, where she has managed the Mass Cytometry Center of Excellence since its inception in 2017. As director of the Cancer and Immunology Core at Vanderbilt, her focus is on increasing both the standardization and accessibility of cutting-edge cytometry techniques for application across a broad range of basic and translational research projects.
Webinar Summary This webinar aims to give an experience-driven overview of considerations for choosing the appropriate high dimensional flow cytometry technology for a project as well as guidance for those who have experience in one technology and are transitioning to the other.
Learning Objectives Attendees will leave the webinar with a strong foundation in high dimensional cytometry principles and applications, as well as sufficient working knowledge to select the appropriate technology for their study.
Who Should Attend: Users who are interested in high-dimensional cytometry of any kind.
A CYTO U Webinar presented by Kylie Price
Keywords: Shared Resource Laboratories, team science, health research culture, equality and diversity
About the Speaker
Kylie Price Chief Technology Officer New Castle University Malaghan Institute of Medical Research
Kylie Price is the Chief Technology Officer at the Malaghan Institute of Medical Research with 20 years’ experience providing strategic, scientific, and operational direction in multidisciplinary environments. Kylie has strong stakeholder management and engagement skills and has attracted more than $35m of philanthropic funding over the past 12 years, supporting the creation of a distinguished centre of research excellence, the Hugh Green Cytometry Centre (HGCC). Kylie leads a team of twelve highly skilled staff scientists in providing support to over 90 scientists at the Malaghan Institute. The HGCC provides access to cutting-edge technology platforms (including full spectrum flow cytometry, histology, bioimaging, data science, molecular biology and mRNA for pre-clinical vaccine development), and she advises multiple research groups and companies both nationally and abroad. Kylie also has a strong leadership track-record, organizing high-profile networking events, such as CYTOAsia Singapore 2017, and directing international organizations, such as the Australasian Cytometry Society of which she is former President (2015-2016). Kylie is a two-time NZ Woman of Influence Awards (2014 and 2018) finalist and finalist of the 2021 NZ High Tech Awards. She was the first New Zealander elected to the International Society for Advancement of Cytometry (ISAC) Council (2018-2020) and is currently serving as Chair of the Governance Committee and Secretary of ISAC (2020-2024). In April 2023 Kylie was selected to join the NZ OnBoard Program and is currently a board observer for Orbis Diagnostics.
Webinar Summary
This webinar will cover an overview of Kylie’s career path and provide examples of how she promotes equity, employee engagement and participation within her team. She will cover the SRL Career Progression Framework she developed with others at the Malaghan Institute of Medical Research and discuss how she encourages the appropriate recognition of SRLs and SRL staff scientists in publication. There will be a Q+A session and we hope for lots of questions and engagement.
Learning Objectives
1. New ideas for how to increase SRL staff engagement and participation 2. A guideline to follow for the appropriate recognition of SRLs and SRL staff scientists in publication 3. An example of a non-traditional career path to Chief Technology Officer 4. Strategies for promoting equity in the workplace
Who Should Attend: Scientists who manage teams (PIs, researchers, SRL managers), SRL staff scientists, women in STEM, scientists interested in topics related to equity and inclusion.
Use of Spectral Flow Cytometry in Clinical Trials By Veronica Nash and Tania Nevers
Veronica Nash, PhD US Regional Head of Flow Cytometry Cerba Research
Veronica earned her B.Sc and M.Sc in Medical Biotechnologies from “Tor Vergata” and from “Sapienza” University, respectively, in Rome, Italy. She continued her training at Ghent University, Belgium where she received her PhD in Medical Sciences. After her PhD, she joined Icahn School of Medicine at Mount Sinai in NYC where she leveraged her knowledge in virology and immunology with her Flow Cytometry expertise to identify host cofactors in the immune cell which are involved in HIV and HTLV infection. Afterwards, she moved to Indiana University in Bloomington, IN, and as a staff scientist she optimized a Flow Cytometry method to identify protein mutants to be used as a target in precision medicine for Cystic Fibrosis. Veronica’s career in industry started at Wuxi Apptec where she developed Flow Cytometry assays for QC of drug therapy products, such as CAR-T cells and TILs. Then she joined Labcorp Drug development (Covance) as a staff scientist where she focused on Flow cytometry assay validation, data review and instrument implementation. Currently, as the US Regional Head of Flow Cytometry department at Cerba Research, she is the scientific lead for high-parameters Flow Cytometry; in the last year, she led a global team through the implementation and harmonization of Cytek Aurora instruments across multiple sites. Veronica is also an active member of ISAC and ICCS; currently she is the leader of a working group which aims at promoting CLSI H62 guidelines education within the Flow Cytometry community.
Tania Nevers Senior Principal Scientist Bristol Myers and Squibb
Tania earned her B.Sc and M.Sc in Biology from St John’s University, Queens NY before receiving her PhD in Biomedical Sciences from Brown University in Providence, RI. Following her Ph.D., Tania pursued a postdoctoral training at Tuft’s University where her work combined areas of immunology, vascular biology and cardiac physiology to study several aspects of adaptive immunity in diverse inflammatory settings. She then moved on to a Contract Research Organization, Flowmetric Inc, where she served as a Scientist and then Project Manager. Her primary responsibility was to design, review and execute complex flow cytometry experiments in both preclinical and clinical studies for client sponsored projects. She then transitioned to the large Pharmaceutical Industry where she currently resides as a Senior Principal Scientist at Bristol Myers and Squibb. She primarily works on high order assay development, validation and implementation, and is the subject matter expert for Cytek Aurora implementation across BMS, including integration and harmonization activities.
When not at work, Tania enjoys spending quality time with family as well as listening to music, dancing and gardening.
About the webinar:
The use of Spectral Flow Cytometry allows for a deeper characterization of immune subsets (immune profiling) than conventional flow cytometry and is more valuable with limited patient samples in biomarker discovery in drug development. This webinar describes how to implement and standardize Cytek Aurora instruments and how to design, develop, and validate 30+ parameters panels that can be successfully utilized in clinical trials.
Learning Objectives:
• Learn how to implement and standardize Cytek Aurora instruments • Understand how to validate an assay for 30+ colors on a spectral instrument to use in clinical trials • Learn solutions on challenges such as reference controls, unmixing strategies and experimental sept up when implementing a high-parameter assay for global clinical trials
