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Products are filtered by different dates, depending on the combination of live and on-demand components that they contain, and on whether any live components are over or not.
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  • Contains 3 Component(s), Includes Credits

    Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox Keywords: Flow Cytometry, Cancer Research, Radiotherapy, Shared Resource Laboratory, Instrumentation

    Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox

    About the Presenter

    image

    Dagna Sheerar
    Flow Cytometry Director
    University of Wisconsin - Madison
    Carbone Cancer Center Flow Cytometry Laboratory

    Dagna Sheerar, SCYM(ASCP)CM, has been working in flow cytometry shared resource laboratories for over 20 years and is currently the director of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow).  UWFlow is a busy core with a staff of 7 full time flow cytometrists, including Dagna, with 2 core laboratory locations on campus.  Dagna’s areas of interest are core administration and management, rigor and reproducibility, and user education. Since becoming director, Dagna has successfully added, on average, one instrument per year to the Flow Lab through various grant programs, institutional purchases, or “crowd funding.” Dagna has been involved in the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) for almost as long as she has been working in cores, and she recently served as GLIIFCA president from 2020-2021 and currently serves on their Board of Directors. She is a member of ISAC and serves on the SRL Content Task Force. She serves as host, organizer, and instructor for the Annual Course in Cytometry, and recently completed the 45th Annual Course held at the University of Wisconsin in June of 2022. Dagna has presented at GLIIFCA, the annual CYTO Meeting, and the Association of Biomolecular Resource Facility (ABRF) annual meetings. Dagna also served as the Interim Director of Campus Research Cores at UW – Madison in 2020.  

    image

    Kathryn Fox
    Flow Lab Technical Manager
    University of Wisconsin
    Carbone Cancer Center Flow Cytometry Laboratory

    Kathryn Fox, SCYM(ASCP)CM, is currently the technical manager of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow). She has been working for the UW Carbone Cancer Center Flow Cytometry Core Laboratory since 2015. Her flow cytometry interests include digging around inside instruments, any application that involves staining DNA, and being surprised by interesting new projects brought forward by the core’s diverse user base. Kathryn graduated from Iowa State University with a BS in Biology and then from the University of Wisconsin – Madison with a PhD in Cancer Biology. She was first introduced to flow cytometry in graduate school, but her main focus at that time was fluorescence microscopy. After graduate school, she worked as an applications scientist in multiphoton microscopy. Since diving into flow cytometry, Kathryn has been an active participant in GLIIFCA and the Annual Course in Cytometry, and finally got to attend her first in-person CYTO in 2022. 

    Webinar Summary

    This seminar will focus on how a flow core can respond to the needs of researchers to analyze radiotherapy treated samples in real time via flow cytometry.  We will present our process for providing this service from the initial idea, partnering with our Small Animal Imaging and Radiotherapy Facility, working with our institutional Radiation Safety group to test various cleaning methods for removing radioactive particles from the instrument post-analysis, instrument modifications, working with the original equipment manufacturer to ensure compliance with our existing service contract and safety for the engineers servicing the equipment, and additional safety measures and training procedures developed specific to this service.


    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- A Novel Cytometry Analysis Framework In Python: Application to Real-World Immunophenotyping of Patients With Severe Sepsis

    Speakers: 

    Ross Burton

    Simone Cuff

    Andreas Artemiou

    Matthias Eberl

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- High-Throughput Image-Activated Cell Sorting By Mitochondrial Relocation

    Speakers: 

    Maik Herbig

    Akihiro  Isozaki

    Jeffrey Harmon

    Keisuke  Goda

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- High-Speed Image-Based Cell Sorting

    Speakers: 

    Eric Diebold

    BD CellView Team

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- Functional Single-Cell Proteomics of Glioblastoma Defines Extensive Drug Response Heterogeneity and Therapy-Induced Cellular Plasticity

    Speakers: 

    Dena Panovska

    Asier Antoranz

    Steven De Vleeschouwer

    Frederik De Smet

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- mRNA Vaccine-Induced T Cells Transition to a Resting Memory State Capable of Rapid Proliferation and Differentiation to Effector Lineages Upon Antigen Re-Exposure

    Speakers: 

    Mark Painter

    John Wherry

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- Uncovering the Pathogenesis and Treatment of Atherosclerosis By High-Throughput Microscopy

    Speakers: 

    Yunjie Deng

    Jaime Alvarez Duque

    Chengxun Su

    Yuqi Zhou

    Masako Nishikawa

    Ting-Hui Xiao

    Yutaka Yatomi

    Han Wei Hou

    Keisuke Goda

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Plenary- Cytometry as a Service

    The Role of Big and Small CROs in the Cytometry Ecosystem
    Virginia Litwin, Charles River Laboratories
    Julie Bick, FlowMetric Life Sciences Inc
    Vilma Decman, GlaxoSmithKline
    Cherie Green, Ozette
    Nithianandan Selliah, Cerba Research


    The Specialty CRO:
    Making New Technology Accessible
    Ryan Brinkman, CytaPex/Insightful Science
    Pratip Chattopadhyay, Talon Biomarkers
    John Nolan, Cellarcus Biosciences
    John O'Rourke, BennuBio

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Workshop- Engaging the un-Engaged and How to Keep Them

    Lead Speaker: Gelo De la Cruz

    Background
    Gelo dela Cruz is the Flow Cytometry Platform manager at the Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) in Copenhagen, Denmark. Prior to reNEW, Gelo worked at the flow cytometry core facilities of the German Cancer Research Center and the New York University Cancer Institute. His work in the past 10 years has been in developmental biology and stem cell research and has been co-author and acknowledged in several publications. Gelo has been an active ISAC member and a CYTO Conference attendee since 2011 and was an ISAC SRL Emerging Leader (2015-2019). He has served in different committees (Education Committee, Meetings Committee, CYTO Youth, and Associated Societies subcommittees) and currently is the chair of the Education Shared Resource Laboratory Content subcommittee. Gelo is also active in the Danish Society for Flow Cytometry and is the chairperson of the board (2019-2022). Gelo holds a degree in Molecular Biology and Biotechnology and a Specialist in Cytometry certification, SCYM(ASCP), from the American Society for Clinical Pathology. 

    Additional Speakers: Alfonso Blanco and Lourdes Arriaga-Pizano

    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Workshop- Consensus Best Practices for User Consultations in an SRL

    Lead Speaker: Michael Gregory

    Background
    Mike is the Flow Cytometry Core Director at Cleveland Clinic’s Florida Research and Innovation Center in Port St. Lucie, FL. He is ASCP SCYM-certified, a past ISAC SRL Emerging Leader and has worked in flow cytometry SRLs for 18 years. As the current Chair of the ISAC SRL Committee, he has been committed to the continued development of ISAC’s SRL Best Practices and helping all SRLs attain the higher level of excellence outlined in those Best Practices. 

    Additional Speakers: Amy Graham and Kewal Asosingh

    CMLE Credit: 1.0

    • Register
      • Visitor - Free!
      • Bronze - Free!
      • Silver - Free!
      • Gold - Free!
      • Platinum - Free!
      • Community Administrator - Free!
      • ISAC Staff - Free!
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