21-Color Flow Cytometry for Immunophenotyping Human Blood
Recorded On: 03/27/2018
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About the Presenter
Karl Staser, MD, PhD
Ascension Medical Group
Dr. Karl Staser graduated with a BA in American History and Literature from Harvard College in 2002. He then enrolled in the Indiana University School of Medicine, where he completed a Howard Hughes Medical Institute “year-out” research fellowship for medical students and subsequently entered the Indiana University Medical Scientist Training Program under the supervision of Dr. Wade Clapp. Dr. Staser then joined the Physician Scientist Training Program at Washington University and completed his internal medicine internship and dermatology residency at Barnes-Jewish Hospital in 2017. He is now researching novel therapies for graft-versus-host disease and cutaneous T cell lymphoma in Dr. John F. DiPersio’s lab at the Siteman Cancer Center at Washington University in St. Louis.
The 21-color flow cytometry panel enables simultaneous quantification of monocytes, basophils, granulocytes, dendritic cells, natural killer cells, B cells, and all well-defined T and T helper cell subsets in the human peripheral blood. This panel captures the major phenotypes described in the NIH Human Immunology Project with additional markers for deep T cell analysis. We specifically designed this panel for peripheral blood analysis from patients involved in our clinical trials of novel agents for the treatment of graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (alloHSCT). We have optimized this panel for the analysis of 1x10^6 fresh or previously frozen peripheral blood mononuclear cells (PBMCs).
- Describe the process of designing and validating a 21-color flow cytometry panel.
- Demonstrate the application of this particular 21-color flow cytometry panel.
- Discuss troubleshooting and experimental refinement of 21-color flow cytometry.
Who Should Attend.
Physicians, scientists, and other researchers interested in high-color flow cytometry for the rapid and reproducible immunophenotyping of human blood.
CMLE Credit: 1.0