A Beginner's Guide to Computational Cytometry: From Algorithms to Applications

With the increase in panel sizes, more and more people are interested in computational cytometry tools to supplement their manual gating strategies. In this tutorial, I will give an overview of the typical computational cytometry pipeline we use in our lab. I will introduce several types of algorithms, what their strengths and caveats are and in which situations they might be applicable. The algorithms will be demonstrated both on a toy dataset, to showcase the principles and which will be made available for further exploration by the participants, as well as on concrete examples of studies we worked on in our lab. At the end, the audience should have a clear idea of some computational tools that might be worth trying on their own use cases.

CMLE Credit: 1.0