Advancing 2D and 3D Spectral Cytometry: Principles, Best Practices, and Applications of High-Multiplex Tissue Imaging

The Speakers

Andrea J. Radtke, Ph.D. - Principal Scientist, Spatial Biology - Leica Microsystems

Andrea J. Radtke, Ph.D. specializes in advanced microscopy techniques including IBEX, a multiplexed antibody-based imaging method she co-developed with her colleagues at the National Institutes of Health. Dr. Radtke has used IBEX to create multi-omic atlases of normal and malignant human lymphoid organs for discovery and translational research. Dr. Radtke is passionate about open science and co-leads several community efforts within the field of spatial biology including the IBEX Imaging Community and Organ Mapping Antibody Panel initiative of the Human BioMolecular Atlas Program. Dr. Radtke serves as a Principal Scientist at Leica Microsystems, where she remains dedicated to advancing discovery through innovations in spatial biology.

Michael Smith, Ph.D. - Applications Manager - Leica Microsystems

Michael Smith, Ph.D. is Application Manager at Leica Microsystems. He completed a Ph.D. in the genetics of DNA repair at Columbia University and a postdoctoral fellowship in genome integrity and cellular aging at Memorial Sloan Kettering Cancer Center, where he leveraged microscopy to understand the real-time dynamics of DNA homeostasis. His passion for answering biological questions using imaging technologies led him to his current position, where he provides technical expertise and customer support for Leica’s line of spatial biology solutions.

Webinar Summary

Imaging-based spatial omics has enabled unprecedented characterization of cellular interactions and spatial relationships within tissues. Within the last decade, the field has seen a rapid expansion of spatial biology methods employing fluorescently conjugated probes to identify and localize a high number (>10) of proteins or RNA molecules in a single specimen. Recent advances, such as those seen in spectral cytometry and novel dye chemistries, now permit high-multiplex imaging in both 2D and 3D contexts. This webinar will highlight application areas of spectral imaging including 3D atlas construction and immunophenotyping of diverse tissues. Additionally, we will share foundational knowledge, practical tools, and key resources to empower the ISAC community to adopt cutting-edge spectral imaging in their research and core facilities.

Learning Objectives

1. Overview of multiplexed imaging: Participants will learn about common challenges and solutions for high-multiplex imaging.

2. Designing panels: Participants will gain insights into designing panels for 2D and 3D high-multiplex imaging using parallels from flow cytometry and domain specific knowledge related to spatial proteomics.

3. Selecting controls: Participants will learn how to design appropriate controls including single and group controls for spectral unmixing.

4. Advances in the field: Participants will learn about cutting-edge spectral unmixing technologies and AI-powered image analysis.  

Who Should Attend

This webinar is intended for all current and potential spatial biology enthusiasts (novice to expert) interested in performing high-multi-plex 2D and 3D imaging

CMLE Credit: 1.0