Best Practices for Preparing a Single Cell Suspension from Solid Tissues
About the Speaker
Dr. Kewal Asosingh
Scientific Director for Flow Cytometry
Cleveland Clinic Lerner Research Institute
Dr. Asosingh is a principal investigator and scientific director for flow cytometry at the Cleveland Clinic Lerner Research Institute. He is a past ISAC Scholar, Cytometry Part A associate editor, ISAC Flow Cytometry Content subcommittee chair, and member of the Education Committee. He has more than 20 years of experience in solid tissue disaggregation into a single-cell suspension.
A good single-cell prep is essential for any flow cytometry and single-cell omics experiment. This webinar outlines the principles and provides a general guide of steps to consider for solid tissue disaggregation.
- Basics of solid tissue composition.
- What is a “good” single-cell prep in flow cytometry and single-cell RNAseq experiments?
- The dos and don’ts in designing a tissue disaggregation protocol.
- Evaluation of the quality of a single cell prep and common pitfalls.
Who Should Attend
Flow cytometry SRL staff and anyone performing flow cytometry using solid tissue as starting material.
CMLE Credit: 1.0