A CYTO U Webinar presented by Michael Milone, PhD
Keywords: tumor immunology
About the Presenter
Michael Milone, PhD Associate Professor of Pathology and Laboratory Medicine Associate Director, Toxicology Laboratory University of Pennsylvania Perelman School of Medicine
Dr. Michael Milone received his MD and PhD in experimental pathology in 1999 from New Jersey Medical School. After an internship in internal medicine and post-graduate medical training in clinical pathology and transfusion medicine at the Hospital of the University of Pennsylvania, he pursued a post-doctoral research fellowship with Dr. Carl June at the University of Pennsylvania, where he studied T cell immunotherapy for cancer and designed the CD19-specific CAR-T cell therapy that became CTL019 (tisagenlecleucel, KymriahTM), the first FDA-approved gene therapy in the US. He is a founding member of the Center for Cellular Immunotherapies at the University of Pennsylvania. He currently directs a research laboratory focused on basic and translational immunology that includes the design and application of synthetic immunoreceptors for adoptive cellular therapy of cancer and antibody-mediated disease.
Webinar Summary
This webinar will provide an overview of engineered T cell adoptive immunotherapies that utilize synthetic immunoreceptors termed chimeric antigen receptors (CARs). Topics to be covered include the design of CAR-T cells, approaches to preclinical assessment of efficacy and toxicity, and the clinical application of this technology to the treatment of malignant and autoimmune disease.
Learning Objectives
Understand the design and mechanism of action of engineered T cell adoptive immunotherapies using chimeric antigen receptors (CARs).
Describe some of the challenges to applying CAR-T cell therapy to cancer therapy.
Discuss the clinical application of engineered T cells.
Who Should Attend
Scientists, physicians, or technologists with an interest in immunotherapy.
A CYTO U Webinar presented by Alex Walsh, PhD
Keywords: Optical imaging, 2-photon imaging, cancer therapy, Autofluorescence
About the Presenter
Alex Walsh, PhD Assistant Professor in Biomedical Engineering Texas A&M University
Dr. Walsh completed her Ph.D. at Vanderbilt University, where she developed an autofluorescence lifetime-based assay for determining the optimal cancer treatment strategy for individual patients. As a postdoc at the Air Force Research Lab, Dr. Walsh used optical techniques to investigate infrared-light activation and inhibition of action potential propagation in neurons. Currently, Dr. Walsh is an assistant professor in the Biomedical Engineering Department at Texas A&M University.
Webinar Summary
Fluorescence lifetime imaging (FLIM) of the endogenous fluorophores, NAD(P)H and FAD (co-enzymes of metabolic reactions), provides a label-free method to quantify cellular metabolism. This webinar will review multi-photon fluorescence lifetime imaging methods, single cell segmentation, and intra-population heterogeneity analysis. Examples will be shown for drug response in breast cancer organoids and activation of T cells.
Learning Objectives
Define fluorescence lifetime and time-correlated single photon counting imaging methods. Interpret label-free FLIM images of NAD(P)H and FAD.
Discuss segmentation and single-cell analysis techniques.
Who Should Attend
Anyone interested in label-free imaging, fluorescence lifetime imaging, or single-cell analysis.
A CYTO U Webinar presented by Jonathan Irish, PhD
Keywords: CyTOF, Big data, Pipeline, Clustering, FlowSOM, Automated Labeling, MEM
About the Presenter
Jonathan Irish, PhD Assistant Professor Department of Cell and Developmental Biology (CDB) Vanderbilt University
Jonathan Irish is an assistant professor in the Department of Cell and Developmental Biology (CDB) at Vanderbilt University, School of Medicine. He holds a secondary appointment in Pathology, Microbiology, and Immunology (PMI) and is scientific director of the Cancer and Immunology Core (CIC) and the Mass Cytometry Center of Excellence (MCCE). Jonathan launched the Irish Lab at Vanderbilt in 2012 after finishing his training with Garry Nolan and Ron Levy at Stanford University. Jonathan holds a BS in biology, a BS in chemistry, and a BS biochemistry from the University of Michigan. He received a PhD in cancer biology from Stanford University.
A CYTO U Webinar presented by Jonni Moore, PhD
Keywords: Clinical cytometry, computation, cytomics, validation, diagnostic, biomarker
About the Presenter
Jonni Moore, PhD Professor of Pathology and Laboratory Medicine University of Pennsylvania
Dr. Jonni Moore has over 30 years of experience in clinical and research flow cytometry. She has led one of the largest flow cytometry and cell sorting shared resource labs since 1992 and was founding director of the Clinical Flow Cytometry Laboratory at the Hospital of the University of Pennsylvania. Her current focus is on deep phenotyping in translational and clinical settings and the development of liquid biopsies utilizing ultra-sensitive flow cytometry.
In 2016 she received the Wallace H. Coulter Distinguished Lecturer Award for lifetime contributions to the science, education, and practice of clinical cytometry. Being in the unique position of leadership in both clinical and research flow cytometry laboratories, she focuses on the movement of novel translational assays to the clinical arena.
Webinar Summary
This webinar will focus on the recent new technologies that are expanding the reach of translational and clinical cytometry, not only in traditional areas, but also in new areas like solid tumors and cardiovascular diseases. The power of the technology to provide important information in these settings will be highlighted as well as the necessity for biocomputational approaches for both current and developing applications.
Webinar Objectives
To highlight new technologies that can extend the reach of clinical cytometry.
To highlight new disciplines that are emerging in clinical cytometry.
To introduce the role of biocomputation in the clinical realm.
To stimulate interest in the opportunities for development in the translational/clinical area.
Who Should Attend this Webinar
Clinicians, lab directors, technologists, and scientists who are interested in translational and clinical cytometry.
A CYTO U Webinar presented by Anja E. Hauser, PhD
Keywords: multiplex histology, quantitative, bone marrow
About the Presenter
Anja Hauser, PhD Professor of Immune Dynamics and Intravital Microscopy Charité—Universitätsmedizin and Deutsches Rheumaforschungszentrum
During her studies of veterinary medicine, Anja Hauser developed an interest in immunopathology and microscopy. As a graduate student, she identified factors which attract plasma blasts into the bone marrow and keep them alive in specialized niches within this tissue. During her postdoctoral work, she worked on the migration of germinal center B cells using intravital 2-photon microscopy. She is a professor of immune dynamics and intravital microscopy at the Charité—Universitätsmedizin and Deutsches Rheumaforschungszentrum in Berlin. In an interdisciplinary approach, her lab develops novel microscopy technologies to obtain a deeper insight in how the immune system functions.
Webinar Summary
Anja will introduce the principles of multi-epitope ligand cartography (MELC), a method for multiplexed immunofluorescence microscopy, and explain its application for analyzing complex tissues and rare cell subsets. She will also give an overview on methods suitable to quantitatively analyze those complex multi-parametric image data.
Learning Objectives
Describe MELC as a method for multiplexed immunofluorescence histology.
Learn what to consider when preparing tissues for MELC analysis, such as choosing marker panels and planning the sequential staining in the tissue
Learn about options for image analysis in order to extract quantitative information from multiplexed immunofluorescence histology.
Who Should Attend
Everyone who is interested in quantitative multiplexed image analysis and its applications in immunology.
A Cytometry Part A Spotlight CYTO U Webinar presented by Karl Staser, MD, PhD
Keywords: OMIP, phenotyping, panel design, PBMCs, T cells, human immunology
About the Presenter
Karl Staser, MD, PhD Ascension Medical Group
Dr. Karl Staser graduated with a BA in American History and Literature from Harvard College in 2002. He then enrolled in the Indiana University School of Medicine, where he completed a Howard Hughes Medical Institute “year-out” research fellowship for medical students and subsequently entered the Indiana University Medical Scientist Training Program under the supervision of Dr. Wade Clapp. Dr. Staser then joined the Physician Scientist Training Program at Washington University and completed his internal medicine internship and dermatology residency at Barnes-Jewish Hospital in 2017. He is now researching novel therapies for graft-versus-host disease and cutaneous T cell lymphoma in Dr. John F. DiPersio’s lab at the Siteman Cancer Center at Washington University in St. Louis.
Webinar Summary
The 21-color flow cytometry panel enables simultaneous quantification of monocytes, basophils, granulocytes, dendritic cells, natural killer cells, B cells, and all well-defined T and T helper cell subsets in the human peripheral blood. This panel captures the major phenotypes described in the NIH Human Immunology Project with additional markers for deep T cell analysis. We specifically designed this panel for peripheral blood analysis from patients involved in our clinical trials of novel agents for the treatment of graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (alloHSCT). We have optimized this panel for the analysis of 1x10^6 fresh or previously frozen peripheral blood mononuclear cells (PBMCs).
Learning Objectives
Describe the process of designing and validating a 21-color flow cytometry panel.
Demonstrate the application of this particular 21-color flow cytometry panel.
Discuss troubleshooting and experimental refinement of 21-color flow cytometry.
Who Should Attend.
Physicians, scientists, and other researchers interested in high-color flow cytometry for the rapid and reproducible immunophenotyping of human blood.
A CYTO U Webinar presented by Thomas Cimato, MD, PhD
Keywords: cardiovascular disease, myocardial infarction, hematopoietic stem cells, atherosclerosis progenitor cells, nutrition, ovarian cancer, peripheral blood mononuclear cells, monocyte, macrophages
About the Presenter
Thomas Cimato, MD, PhD Associate Professor University at Buffalo Jacobs School of Medicine and Biomedical Sciences
Dr. Cimato is an associate professor in the Division of Cardiology at the University at Buffalo Jacobs School of Medicine and Biomedical Sciences and an adjunct investigator at the Roswell Park Cancer Institute.
His research focuses on the role of inflammation on hematopoietic differentiation in atherosclerosis. He is also involved in collaborative studies that focus on the role of hematopoietic progenitor cells as immune suppressor cells in cancer.
Webinar Summary
In this webinar he will discuss recent advances in our understanding of how early hematopoietic progenitors respond to hypercholesterolemia and myocardial infarction in animal models to accelerate atherosclerosis. He will discuss the development of an assay to measure human hematopoietic progenitors in blood and early translational studies in human subjects with heart disease.
A CYTO U Webinar presented by Chris Bakal, PhD
Keywords: Cell shape, RNAi screen, Morphological signatures, Cell Morphogenesis, Clustering, Drosophila, Melanoma
About the Presenter
Chris Bakal, PhD Dynamical Cell Systems Team Leader Division of Cancer Biology at the Institute of Cancer Research
Webinar Summary
The Dynamical Cell Systems team is using high throughput functional genomic approaches in tandem with integrative computational technologies to understand the architecture and dynamics of signal transduction networks. They study the biological switches that cause cells to change shape, become cancerous, and spread around the body. By understanding how these switches work, scientists may one day find a way to control them through drugs or other therapies. Cells are able to assume a wide variety of complex shapes in order to carry out different roles, and Dr. Bakal's team examines the genetic and biochemical mechanisms that underpin these shape changes. Their research covers how both environmental and genetic variation affects cell shape. Ultimately, Dr. Bakal aims to understand how normal and cancerous cells can adopt different shapes and why metastatic cancer develops in some people but not others.
A CYTO 2019 State-of-the-Art Lecture Presented by Atul Butte, University of California, San Francisco
Keywords: Precision immunology, Color Wheel, Panel Design, CYTOBrute, Clustering, Immunotherapy, Nivolumab, Ipilimumab, Machine Learning, Elastic Net
The Presenters
A. Butte University of California, San Francisco
Session Summary
There is an urgent need to take what we have learned in our new “genome era” and use it to create a new system of precision medicine, delivering the best preventative or therapeutic intervention at the right time, for the right patients. Dr. Atul Butte's lab at the University of California, San Francisco builds and applies tools that convert trillions of points of molecular, clinical, and epidemiological data—measured by researchers and clinicians over the past decade and now commonly termed “big data”—into diagnostics, therapeutics, and new insights into disease. Several of these methods or findings have been spun out into new biotechnology companies.
Dr. Butte, a computer scientist and pediatrician, will highlight his lab’s recent work, including the use of publicly-available molecular measurements to find new uses for drugs including new therapies for autoimmune diseases and cancer, discovering new druggable targets in disease, integrating, and reusing the clinical and genomic data that result from clinical trials, and how the next generation of biotech companies might even start in your garage. In particular, Dr. Butte will describe the public data resources in the NIAID ImmPort available to study immunology, infection, vaccination, and transplantation, and how these resources can be used to better target drugs and understand immunity across tens of thousands of individuals.
A CYTO 2019 State-of-the-Art Lecture presented by Gustavo Rohde, University of Virginia School of Medicine
The Presenter
Gustavo Rohde University of Virginia School of Medicine
Session Summary
Cell image classification methods are currently being used in numerous applications in cell biology and medicine. Applications include understanding the effects of genes and drugs in screening experiments, understanding the role and sub-cellular localization of different proteins, as well as diagnosis and prognosis of cancer from images acquired using cytological and histological techniques. We review three different approaches for cell image classification: numerical feature extraction, end-to-end classification with neural networks, and transport-based morphometry. In addition, we provide comparisons of four different cell imaging datasets to highlight the relative strength of each method.
Contains 4 Component(s), Includes Credits
Recorded On: 07/11/2018
Contains 3 Component(s), Includes Credits
Recorded On: 11/19/2019
Contains 4 Component(s), Includes Credits
Recorded On: 10/20/2017
Contains 4 Component(s), Includes Credits
Recorded On: 11/15/2017
Contains 3 Component(s), Includes Credits
Recorded On: 04/15/2019
Contains 4 Component(s), Includes Credits
Recorded On: 03/27/2018
Contains 4 Component(s), Includes Credits
Recorded On: 09/27/2016
Contains 4 Component(s), Includes Credits
Recorded On: 03/20/2014
Contains 3 Component(s), Includes Credits
Recorded On: 06/22/2019
Contains 3 Component(s), Includes Credits
Recorded On: 06/22/2019
