Clinical Application of Flow Cytometry to Study Intracellular Processes in Health and Disease

About the Speaker

David Hedley, Ph.D - Emeritus Professor of Medicine and Senior Scientist, University of Toronto and Princess Margaret Cancer Centre

Dr. David Hedley is a physician/scientist, trained in medical oncology and wet lab research at the Royal Marsden Hospital, UK 1975-81. Attending physician in medical oncology and laboratory scientist, Royal Prince Alfred Hospital/Ludwig Institute for Cancer Research, Assistant Professor of Medicine, University of Sydney, 1981-1989. Since 1990 he has held dual appointments at Princess Margaret Hospital, Toronto, as medical oncologist, specializing in breast and gastrointestinal cancers, and senior scientist with a laboratory program focused on cancer biology and experimental treatment. This laboratory program made extensive use of flow cytometry under my direct supervision, studying laboratory models of cancer, as well as interfacing to a large clinical trials program using novel analytical methods for patient monitoring.  

Contributions to Flow Cytometry

Member of ISAC since 1982, serviedon Council 1989-1992, chair of Site Selection Committee, Education and Scientific Advisory Committees. Cytometry Editorial Board 1986-2024. ISAC Fulwyler Awardee 2014 for innovative excellence.  Annual Flow Cytometry Methods Course faculty 1999-2019

Webinar Summary

This Webinar will discuss the application of flow cytometry to study dynamic processes that regulate cell function in health and disease. Major focus is on intracellular signaling and patient monitoring during treatment with targeted agents. Although emphasis is on cancer treatment, the Webinar also considers intracellular signaling pathways that regulate cytokine production in response to infectious agents. I will outline technical aspects of intracellular antigen measurements by flow cytometry, including a general approach to the study of dynamic post-translational modifications, experimental design, and integration into mechanism-based clinical trials of novel agents

Learning Objectives

1) How to do good quality intracellular staining while maintaining light scatter and surface antigens (not as difficult as you might think).
2)  The huge range of antibodies to structural and functional intracellular antigens, most of which have never been tested in flow.  Provided you have a clear question and trustworthy controls, they provide the opportunity for innovative research projects.
3) A general approach to the study of dynamic intracellular processes in live cells, using antibodies to posttranslational modifications such as phosphorylation. Emphasis on live cell function, including dose/response and time course studies. Analytical power when applied both to basic research projects, and to patient monitoring in clinical trials.

Who Should Attend

This webinar should be of broad interest both to the research and the clinical flow communities, especially the curiosity-driven, and to students and supervisors looking for innovative projects.  

CMLE Credit: 1.0