Considerations for Integrating Flow Cytometry with Single Cell Transcriptomics
Recorded On: 06/11/2016
Pratip Chattopadhyay, PhD
NYU Langone Health
In recent years, technical advances have made it possible to obtain transcriptomic data (i.e., mRNA expression) from single cells. This presentation will be focused on using this technology for studies of T cells and will discuss: a) stimulation of cells; b) purification and sorting of single cells; c) considerations for selecting mRNA targets and qualifying assay primers/probes; d) procedures and workflow for performing the assay; and e) data analysis considerations. We will also discuss the power and limitations of transcription-based approaches to cytometry.
- Overview of why transcriptomics might be valuable in cytometry.
- Methods for stimulation of cells.
- Purification/sorting of cells.
- QC of assay and performing pre- and on-chip amplifications.
- Data analysis.
CMLE Credit: 1.5