Endothelial Cell Immunophenotyping Using Flow Cytometry

About the Speaker

Kewal Asosingh, PhD
Scientific Director
Cleveland Clinic Lerner Research Institute

Dr. Asosingh is a principal investigator and scientific director for Flow Cytometry at the Cleveland Clinic Lerner Research Institute. He is a past ISAC Scholar, Cytometry Part A Associate Editor, ISAC Flow Cytometry Content, and Education Committee member. His flow cytometric research is centered on lung vascular biology.

Webinar Summary

Endothelial cells are cells that reside in tissues and circulate in the bloodstream in small numbers. Immunophenotyping these cells has several challenges, like how to detect endothelial cells, how to gate them, and how to figure out if they're really endothelial. All of this will be covered in this webinar.

Learning Objectives

• Immunophenotype of murine and human circulating and organ-specific endothelial cells
  
• Endothelial cell gating strategies and common pitfalls
  
• The dos and don'ts of functional endothelial cell characterization

Who Should Attend

Flow cytometry SRL members and anyone interested in endothelial cell flow cytometry.

CMLE Credit: 1.0