Expansion Microscopy: Improving Resolution Through Uniform Specimen Expansion
Recorded On: 05/01/2019
About the Presenter
Paul Tillberg, PhD
HHMI Janelia Research Campus
Paul Tillberg completed a BS in electrical and materials engineering at UC Berkeley before going on to complete his PhD in electrical engineering at MIT, where he worked on technology development for the biological sciences. In the Boyden group, he conceived, designed, and co-led the development of Expansion Microscopy. He is currently a Fellow/Lab Head at the HHMI Janelia Research Campus, where he continues to develop and disseminate the expansion method in addition to other avenues of technology development for biology.
The expansion microscopy method improves the effective resolution of any optical microscope by ~4-fold by uniformly expanding biological specimens. Expansion is achieved by embedding the tissue in an ultra-swellable gel, followed by a few simple processing steps. The method is easy to adopt and well suited as a histology core facility offering, as it is compatible with existing antibody and fluorescent protein labelling protocols without modification.
- Discuss how to do expansion microscopy.
- Describe the variants associated with expansion microscopy.
- Learn the strengths and drawbacks to evaluate expansion microscopy for user specific applications.
Who Should Attend
Any biologist interested in probing the nanoscale structure of fixed tissue. Basic histology and imaging skills are useful for the smooth adoption of expansion microscopy.
CMLE Credit: .75