Flow Cytometer Calibration and Characterization

Recorded On: 06/10/2017

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The Presenters

John Nolan, PhD
Professor
Scintillon Institute

James Wood, PhD
Associate Professor
Wake Forest School of Medicine

David Parks
Director Emeritus Emeritus
Full Spectrum FACS Wizard
Technical Support
Standford University

Session Summary

I. Overview and Objectives

  • Why calibrate? Why characterize?
  • Types of calibrators and standards.

II.   Calibration of the Fluorescence Intensity Scale

  • Photoelectrons.
  • Fluorophores: MESFs and ERFs.
  • Molecules: Antibodies and ligands.

III.  Characterization of Instrument Performance

  • Definitions of detection limit.
  • Measuring detection efficiency (Q) and background (B).
  • Limit of resolution/detection.

IV.  Practical Applications

  • Instrument evaluation and comparison.
  • Routine QC and SRL best practices.
  • Implications for polychromatic panel design.
  • Measurement of small, dim particles.

CMLE Credit: 1.5

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Flow Cytometer Calibration and Characterization
Recorded 06/10/2017
Recorded 06/10/2017 A CYTO 2017 Scientific Tutorial Presented by John Nolan, James Wood, & David Parks
CMLE Evaluation Form
11 Questions
11 Questions CMLE Evaluation Form
Completion Credit
1.50 CMLE credits  |  Certificate available
1.50 CMLE credits  |  Certificate available