Image Cytometry for the Flow Cytometrist: When Image Really is Everything

Image Cytometry for the Flow Cytometrist: When Image Really is Everything

Recorded On: 06/11/2016

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The Presenters

Andrew Filby, PhD
Director of the Newcastle University Cytometry and Single Cell Core Technology Unit
Newcastle University

David Novo
De Novo Software

Session Summary

The word "cytometry" is a fusion of two Greek words, "kytos" meaning "basket/cell" and "metria" meaning to "measure." Cytometry itself can be conducted in many ways, but whatever the chosen approach, it almost always involves measuring the properties of single cells/particles from within large populations with the sole aim of decoding the heterogeneity inherent to all biological systems. 

While non-image-based Flow Cytometry (NIFC) is a powerful, multi-parameter, high-throughput cytometric technology with widespread applications, it cannot provide the spatial/morphometric information often essential to addressing key biological questions. As such, a number of imaged-based cytometric approaches exist that can be grouped into a relatively broad field known as "Imaging Cytometry" (IC). This tutorial is primarily aimed at cytometrists who have a background in NIFC and want to understand the basic principles of IC and how it can be pivotal in decoding biological heterogeneity at the cellular level. 

This session will provide a generic definition and the underlying principles of IC as well as outlining the key stages in a typical IC experimental pipeline. Application examples will be included where appropriate in order to provide context to the participants. A recurring theme throughout the tutorial will be to compare and contrast IC with NIFC in order to provide a foundation for introducing IC as a potentially new concept to the attendees by building on their existing knowledge/experience.

CMLE Credit: 1.5


Image Cytometry for the Flow Cytometrist: When Image Really is Everything
Open to view video.
Open to view video. A CYTO 2016 Scientific Tutorial presented by Andrew Filby, Ph.D. and David Novo
CMLE Evaluation Form
11 Questions
11 Questions CMLE Evaluation Form
Completion Credit
1.50 CMLE credits  |  Certificate available
1.50 CMLE credits  |  Certificate available