One is the Loneliest Number: The Journey of a Single Cell through Cytometry to Genomics to Bioinformatics

One is the Loneliest Number: The Journey of a Single Cell through Cytometry to Genomics to Bioinformatics

Recorded On: 06/10/2017

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The Presenter

P. Rogers, J. Bochicchio, and C. Nusbaum
Broad Institute of MIT and Harvard

Session Objectives

  • To learn the best practices for sorting single cells on different cell sorters for the range of genomics applications.
  • To understand how to set up your facility to facilitate single cell sorting experiments.
  • To understand how post-sorting handling and storage can affect cells in downstream analyses.
  • To recognize warning signs of degraded cells in library construction.
  • To get a view of the most commonly used techniques in single cell analyses, including RNAseq, DropSeq, and 10x.
  • To learn how to troubleshoot when processes fail.

CMLE Credit: 1.5

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One is the Loneliest Number: The Journey of a Single Cell through Cytometry to Genomics to Bioinformatics
Recorded 06/10/2017
Recorded 06/10/2017 A CYTO 2017 Scientific Tutorial Presented by P. Rogers, J. Bochicchio & C. Nusbaum, Broad Institute of MIT and Harvard
CMLE Evaluation Form
11 Questions
11 Questions CMLE Evaluation Form
Completion Credit
1.50 CMLE credits  |  Certificate available
1.50 CMLE credits  |  Certificate available