Proliferation Monitoring by Flow Cytometry: Lesson 3A

Authors

Kylie M. Price
Chief Technology Officer & Hugh Green Fellow
Hugh Green Technology Centre
The Malaghan Institute of Medical Research

Katharine A. (Kathy) Muirhead, Ph.D.
Chief Operating Officer, SciGro, Inc.

Paul K. Wallace, Ph.D.
CSO, SciGro, Inc., Southwest Office
Roswell Park Comprehensive Cancer Center, Professor Emeritus 

Lesson 3 describes the use of dye dilution to monitor cell division, with an emphasis on multicolor methods for detecting differential responses in complex cell populations without the need for manual isolation and counting.  

Module 3A, the first of four modules, focuses on: 

1. Principles and methods for using protein-reactive and/or membrane-intercalating dyes to determine the extent of cell division within a population of interest.
2. Similarities, differences, and considerations for selecting among commercially available proliferation tracking dyes.
3. Critical controls required when using dye dilution to monitor the extent of cell division. 

Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wishes to review) dye dilution-based methods for monitoring cell proliferation by flow cytometry is encouraged to attend.

CMLE Credit: 1.0