Proliferation Monitoring by Flow Cytometry, Lesson 3B Data Analysis Strategies

Authors

Kylie M. Price
Chief Technology Officer & Hugh Green Fellow
Hugh Green Technology Centre
The Malaghan Institute of Medical Research

Katharine A. (Kathy) Muirhead, Ph.D.
Chief Operating Officer, SciGro, Inc.

Paul K. Wallace, Ph.D.
CSO, SciGro, Inc., Southwest Office
Roswell Park Comprehensive Cancer Center, Professor Emeritus 

SUMMARY

Lesson 3: Monitoring Cell Division by Dye Dilution 

Lesson 3 describes the use of dye dilution to monitor cell division, with an emphasis on multicolor methods for detecting differential responses in complex cell populations without the need for manual isolation and counting.

Module 3B, the second of four modules, focuses on methods for quantifying extent of cell division based on dye dilution profiles. These include:

1. Describing qualitative trend(s) in antigen expression across generations.
2. Simple quantitation using standard cytometric software (proliferative fraction, stimulation index).
3. Proliferation profile deconvolution using specialized modeling software (% responders, responder expansion)

Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wishes to review) dye dilution-based methods for monitoring cell proliferation by flow cytometry is encouraged to attend.

CMLE Credit: 1.0