Proliferation Monitoring, Lesson 2B: Data Analysis Strategies

This is the second of four modules of Lesson 2 of the Proliferation course. Module 2B provides an in depth discussion of DNA cell cycle data analysis strategies. The underlying concepts behind the characteristic cell cycle distribution are described followed by a description with examples of simple and complex approaches to modeling normal and abnormal DNA fluorescence data.

The course concludes with a discussion of controls to include in proper experimental design and explains how to analyze synchronized cell populations as seen when cell cultures are drug treated. Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wishes to review) how DNA flow cytometric data is analyzed is encouraged to attend.

Authors

Kylie M. Price
Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand

Katharine A. (Kathy) Muirhead 
SciGro, Inc., Midwest Office
Middleton, Wisconsin, USA

Paul K. Wallace  
Department of Flow and Image Cytometry
Roswell Park Cancer Institute
Buffalo, New York, USA

CMLE Credit: 1.0