Proliferation Monitoring, Lesson 2C: Practical Considerations & Troubleshooting

This is the third of four modules in Lesson 2 of the proliferation course, which focuses on cell cycle analysis by flow cytometry. Module 2C: “Practical Considerations and Troubleshooting" examines how choices made during sample preparation, reagent selection, instrument setup, and data collection impact the reliability and quality of the resulting DNA histograms. Topics covered include instrument linearity, fixation effects, doublet discrimination techniques, and special considerations for DNA cell cycle analysis using vital DNA dyes and thymidine analogs.

Authors

Kylie M. Price  
Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand

Katharine A. (Kathy) Muirhead 
SciGro Inc., Midwest Office
Middleton, Wisconsin, USA

Paul K. Wallace  
Department of Flow and Image Cytometry
Roswell Park Cancer Institute
Buffalo, New York, USA

CMLE Credit: 1.0