Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox

Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox

About the Presenter

Dagna Sheerar
Flow Cytometry Director
University of Wisconsin - Madison
Carbone Cancer Center Flow Cytometry Laboratory

Dagna Sheerar, SCYM(ASCP)^CM, has been working in flow cytometry shared resource laboratories for over 20 years and is currently the director of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow).  UWFlow is a busy core with a staff of 7 full time flow cytometrists, including Dagna, with 2 core laboratory locations on campus.  Dagna’s areas of interest are core administration and management, rigor and reproducibility, and user education. Since becoming director, Dagna has successfully added, on average, one instrument per year to the Flow Lab through various grant programs, institutional purchases, or “crowd funding.” Dagna has been involved in the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) for almost as long as she has been working in cores, and she recently served as GLIIFCA president from 2020-2021 and currently serves on their Board of Directors. She is a member of ISAC and serves on the SRL Content Task Force. She serves as host, organizer, and instructor for the Annual Course in Cytometry, and recently completed the 45^th Annual Course held at the University of Wisconsin in June of 2022. Dagna has presented at GLIIFCA, the annual CYTO Meeting, and the Association of Biomolecular Resource Facility (ABRF) annual meetings. Dagna also served as the Interim Director of Campus Research Cores at UW – Madison in 2020.  

Kathryn Fox
Flow Lab Technical Manager
University of Wisconsin
Carbone Cancer Center Flow Cytometry Laboratory

Kathryn Fox, SCYM(ASCP)CM, is currently the technical manager of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow). She has been working for the UW Carbone Cancer Center Flow Cytometry Core Laboratory since 2015. Her flow cytometry interests include digging around inside instruments, any application that involves staining DNA, and being surprised by interesting new projects brought forward by the core’s diverse user base. Kathryn graduated from Iowa State University with a BS in Biology and then from the University of Wisconsin – Madison with a PhD in Cancer Biology. She was first introduced to flow cytometry in graduate school, but her main focus at that time was fluorescence microscopy. After graduate school, she worked as an applications scientist in multiphoton microscopy. Since diving into flow cytometry, Kathryn has been an active participant in GLIIFCA and the Annual Course in Cytometry, and finally got to attend her first in-person CYTO in 2022. 

Webinar Summary

This seminar will focus on how a flow core can respond to the needs of researchers to analyze radiotherapy treated samples in real time via flow cytometry.  We will present our process for providing this service from the initial idea, partnering with our Small Animal Imaging and Radiotherapy Facility, working with our institutional Radiation Safety group to test various cleaning methods for removing radioactive particles from the instrument post-analysis, instrument modifications, working with the original equipment manufacturer to ensure compliance with our existing service contract and safety for the engineers servicing the equipment, and additional safety measures and training procedures developed specific to this service.

CMLE Credit: 1.0