Rapid Comparative Immunophenotyping of Human Mesenchymal Stromal Cells

About the Presenter

Tamara Lekishvili

Dr. Tamara Lekishvili received her PhD in biochemistry from Tbilisi State University, Georgia. Later she was awarded with the NATO scholarship and continued her research at Norwegian University of Science and Technology in neurobiology. After two dynamic post-doctoral positions at the University of Bath and University of Birmingham, she joined the LGC Group, were she is a certified flow cytometry specialist within the cell and molecular biology team. Her major research focus is developing novel cell-based assays using different flow cytometry applications.

Webinar Summary

Standardization of flow cytometry methods is required to support robust and reliable quality assessment of cells during bioprocessing. Fluorescent cell barcoding (FCB) overcomes some of the limitations of conventional flow cytometry methods with improved accuracy and data quality. However, measurement variability across instruments or between analysts remains a challenge that must be addressed.

A modified fluorescent cell barcoding method using fixable viability dye eFlour 506 has been developed that reduces the potential for such variation. Proof of principle has been demonstrated for multiplexed immunophentoypic analysis using cultured human mesenchymal stromal cells (hMSCs) exposed to a variety of bioprocessing conditions during cell expansion.

This approach, which can easily be transferred to a variety of cell types, represents a further step toward achieving the rigor and robustness required for quality assessment of bioprocessing of cell therapy products, allowing more subtle variations in marker expression levels to be confidently observed.

See the original article in Cytometry Part A.

Learning Objectives

• Describe the modified cytosolic fluorescent cell barcoding method.
• Provide the critical technical considerations during the FCB method development.
• Demonstrate the rapid hMSC immunophenotyping utilizing FCB approach.

Who Should Attend

Scientists and researchers interested in FCB by flow cytometry for the rapid and reproducible immunophenotyping.

CMLE Credit: 1.0