Single Cell Analysis of Autofluorescence Lifetime Images

About the Presenter

Alex Walsh, PhD
Assistant Professor in Biomedical Engineering
Texas A&M University

Dr. Walsh completed her Ph.D. at Vanderbilt University, where she developed an autofluorescence lifetime-based assay for determining the optimal cancer treatment strategy for individual patients. As a postdoc at the Air Force Research Lab, Dr. Walsh used optical techniques to investigate infrared-light activation and inhibition of action potential propagation in neurons. Currently, Dr. Walsh is an assistant professor in the Biomedical Engineering Department at Texas A&M University.

Webinar Summary

Fluorescence lifetime imaging (FLIM) of the endogenous fluorophores, NAD(P)H and FAD (co-enzymes of metabolic reactions), provides a label-free method to quantify cellular metabolism. This webinar will review multi-photon fluorescence lifetime imaging methods, single cell segmentation, and intra-population heterogeneity analysis. Examples will be shown for drug response in breast cancer organoids and activation of T cells.

Learning Objectives

• Define fluorescence lifetime and time-correlated single photon counting imaging methods.
  Interpret label-free FLIM images of NAD(P)H and FAD.
• Discuss segmentation and single-cell analysis techniques.

Who Should Attend

Anyone interested in label-free imaging, fluorescence lifetime imaging, or single-cell analysis.

CMLE Credit: 1.0