The Road to a High-Resolution 40-Color Flow Cytometry Immunophenotyping Panel
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About the Presenter
Maria C. Jaimes, PhD
VP Technical Application Support
Cytek Biosciences, Inc.
Dr. Maria Jaimes earned her MD degree at the Universidad Javeriana in Colombia. Dr. Jaimes completed her postdoctoral training at Stanford University in the Department of Microbiology and Immunology. During her postdoc, she focused on characterizing the immune responses to both rotavirus and influenza viruses after natural infection and immunization. In 2005, Dr. Jaimes joined BD Biosciences, where she worked on different aspects of quality assurance and standardization of flow cytometry assays. In 2015, Dr. Jaimes joined Cytek Biosciences. She is part of the R&D team credited for developing the Aurora Full Spectrum Cytometer and has overseen the instrument characterization, verification, and development of multicolor applications. Besides her responsibilities within the R&D team, Dr. Jaimes leads the Technical Applications Support team worldwide.
This webinar will cover OMIP-069 (published in Cytometry Part A in August 2020), the first 40-color fluorescent panel using full spectrum flow cytometry to broadly phenotype much of the cellular composition of the human peripheral immune system. The panel in this OMIP has been thoroughly optimized to ensure high-quality data and well-resolved populations, enabling the description of most canonical subsets of T cells, B cells, NK cells, monocytes, and dendritic cells. Dr. Jaimes will present the journey of the technology, panel design, protocol development, data QC/QA, and data analysis which led to the successful achievement of this 40-color immune profiling panel.
- Understand the concepts behind full spectrum profiling.
- Learn the necessary steps and tools for good panel design.
- Develop expertise on how to troubleshoot and optimize a multicolor panel.
- Learn to recognize high quality vs. compromised data and the potential sources and mitigation of errors.
Who Should Attend
SRL staff/directors, immunologists, CRO staff, and full spectrum flow cytometer users.
CMLE Credit: 1.0