Sony Commercial Tutorial
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Spectral flow cytometers introduced by Sony enable the use of a wider variety of fluorochrome combinations than previously possible with traditional non-spectral systems. The ID7000™ spectral analyzer is the newest addition to the Sony portfolio. In this tutorial, we will highlight some aspects of spectral cell analysis based on examples from the new ID7000 spectral cell analyzer. We will explore the key considerations when designing multicolor panels for use on the new spectral cell analyzer and review the available fluorochromes with respect to their spectral profiles and highlight how similarities and differences between them affect unmixing and data display. We will also share ways to optimize panel design and gain efficiency in the use of controls to ensure the highest data quality when using spectral flow cytometry.