Agenda - Wednesday, August 5th - 8:45am - 9:20pm EDT (GMT-4)
CYTO Innovation Live Technology Showcase & Panel 8:45am - 10:00am
View Session
8:45 - 9:00am
Welcome back to CYTO Virtual 2020!
President's Welcome
Jonni Moore, PhD, Professor of Pathology and Laboratory Medicine; Faculty Director, Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource Laboratory; Executive Director, PathBioresource, Perelman School of Medicine of the University of Pennsylvania
Master of Ceremonies Welcome
Nicole Poulton, PhD, Director, Center for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences
Pratip Chattopadhyay, PhD, Associate Professor of Pathology; Director, Precision Immunology Incubator, Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center
9:00 - 10:00am
CYTO Innovation Technology Showcase
This year's CYTO Innovation Technology Showcase finalists will be selected based on panel judging and audience feedback during a one week pre-CYTO preview period. Each Finalist will present their pitch followed by five minutes of questioning by our Showcase panel regarding the market need, the technological innovation, and the commercialization strategy. Following the Showcase the panel will select one winner, to be announced later in the day.
Session Moderator
John Nolan, PhD, Professor, Scintillon Institute
Showcase Finalists
Andrea Wang, Co-founder & CEO, AHEAD Medicine
Janette Phi, MBA, CCO, Applied Cells
Steve, Tokarz, MBA, CEO, StabiLux Biosciences
Panelists
Diether Recktenwald, PhD, Principal, Desatoya LLC, VP Advanced Technology, (Retired) BD Biosciences
Bill Hyun, MS, PhD, Venture Partner, Genoa Ventures
Elena Holden, MD, President and CEO, Tomocube USA, Inc.
Click here to view this year's semi-finalist video pitch submissions!
Overview
8:45 - 9:00am
Welcome back to CYTO Virtual 2020!
President's Welcome
Jonni Moore, PhD, Professor of Pathology and Laboratory Medicine; Faculty Director, Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource Laboratory; Executive Director, PathBioresource, Perelman School of Medicine of the University of Pennsylvania
Master of Ceremonies Welcome
Nicole Poulton, PhD, Director, Center for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences
Pratip Chattopadhyay, PhD, Associate Professor of Pathology; Director, Precision Immunology Incubator, Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center
9:00 - 10:00am
CYTO Innovation Technology Showcase
This year's CYTO Innovation Technology Showcase finalists will be selected based on panel judging and audience feedback during a one week pre-CYTO preview period. Each Finalist will present their pitch followed by five minutes of questioning by our Showcase panel regarding the market need, the technological innovation, and the commercialization strategy. Following the Showcase the panel will select one winner, to be announced later in the day.
Agenda and Speakers
Session Moderator
John Nolan, PhD, Professor, Scintillon Institute
Showcase Finalists
Andrea Wang, Co-founder & CEO, AHEAD Medicine
Janette Phi, MBA, CCO, Applied Cells
Steve, Tokarz, MBA, CEO, StabiLux Biosciences
Panelists
Diether Recktenwald, PhD, Principal, Desatoya LLC, VP Advanced Technology, (Retired) BD Biosciences
Bill Hyun, MS, PhD, Venture Partner, Genoa Ventures
Elena Holden, MD, President and CEO, Tomocube USA, Inc.
Click here to view this year's semi-finalist video pitch submissions!
Novel UV Lasers for Fluorescence Excitation - Coherent 9:00am - 10:00am
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Instrument manufacturers in the life science market are reporting a new trend. New reagents exposed with UV light are expanding the read capability of their instruments and enable new analysis capabilities of cells. This leads to a more commonly use of UV laser wavelengths in instruments e.g. flow cytometers. Coherent addresses the new trend with a novel platform of OBIS XT UV lasers for the instrumentation market. Further information is given on the specifications and performance of these lasers. Given the nature of the wavelength and the current instrument designs new UV lasers for instrumentation must be disruptive compared with standard DPSS laser technology. The talk reviews how the OBIS XT addresses the design demands from instrument manufacturers and how the integration into instruments like flow cytometers is simplified. The talk will end with recommendations on the integration of UV lasers in general and will end with an outlook into future UV products.
Novel UV Lasers for Fluorescence Excitation
Frank Gäbler, Director of Marketing, Coherent
Overview
Instrument manufacturers in the life science market are reporting a new trend. New reagents exposed with UV light are expanding the read capability of their instruments and enable new analysis capabilities of cells. This leads to a more commonly use of UV laser wavelengths in instruments e.g. flow cytometers. Coherent addresses the new trend with a novel platform of OBIS XT UV lasers for the instrumentation market. Further information is given on the specifications and performance of these lasers. Given the nature of the wavelength and the current instrument designs new UV lasers for instrumentation must be disruptive compared with standard DPSS laser technology. The talk reviews how the OBIS XT addresses the design demands from instrument manufacturers and how the integration into instruments like flow cytometers is simplified. The talk will end with recommendations on the integration of UV lasers in general and will end with an outlook into future UV products.
Agenda and Speaker
Novel UV Lasers for Fluorescence Excitation
Frank Gäbler, Director of Marketing, Coherent
Break 10:00am - 10:30am
View Exhibit Hall
The next session will begin at 10:30 AM. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Overview
The next session will begin at 10:30 AM. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Cutting Edge C: Tools 10:30am - 12:30pm
View Session
A session featuring tools that create bio-molecular design routes, link disciplines and create new views of live systems. Phage display technologies have clinical implications for the production of human and non-human monoclonal antibodies and the isolating of recombinant human and humanized antibody fragments for the design of T-cell chimeric antigen receptors for cancer therapy. Machine learning can stand at the interface of high-throughput immunology and clinical phenotyping. Applying light sheet microscopy and 3D biological imaging technologies, can create an understanding of the biological world from the single cell to tissue-engineered epithelial microtissues.
Session Moderators
Ryan Brinkman, PhD, Professor, Medical Genetics, University of British Columbia
Tomáš Kalina, MD, PhD, Associate Professor, Department of Paediatric Hematology/Oncology, Second Faculty of Medicine, Charles University Prague, Czech Republic
Phage Display Tools for Chimeric Antigen Receptor-T Cell Development
Don Siegel, MD, PhD, Professor of Pathology & Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania
Machine Learning for Integrative Analysis of the Immune System in Clinical Settings
Nima Aghaeepour, PhD, Assistant Professor, Stanford University
Light Sheet Imaging for the Masses
Emmanuel Reynaud, PhD, Principal Investigator, Integrative Biology, Conway Institute of Biomolecular and Biomedical Science, UCD Centre for Biomedical Engineering
Overview
A session featuring tools that create bio-molecular design routes, link disciplines and create new views of live systems. Phage display technologies have clinical implications for the production of human and non-human monoclonal antibodies and the isolating of recombinant human and humanized antibody fragments for the design of T-cell chimeric antigen receptors for cancer therapy. Machine learning can stand at the interface of high-throughput immunology and clinical phenotyping. Applying light sheet microscopy and 3D biological imaging technologies, can create an understanding of the biological world from the single cell to tissue-engineered epithelial microtissues.
Agenda and Speakers
Session Moderators
Ryan Brinkman, PhD, Professor, Medical Genetics, University of British Columbia
Tomáš Kalina, MD, PhD, Associate Professor, Department of Paediatric Hematology/Oncology, Second Faculty of Medicine, Charles University Prague, Czech Republic
Phage Display Tools for Chimeric Antigen Receptor-T Cell Development
Don Siegel, MD, PhD, Professor of Pathology & Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania
Machine Learning for Integrative Analysis of the Immune System in Clinical Settings
Nima Aghaeepour, PhD, Assistant Professor, Stanford University
Light Sheet Imaging for the Masses
Emmanuel Reynaud, PhD, Principal Investigator, Integrative Biology, Conway Institute of Biomolecular and Biomedical Science, UCD Centre for Biomedical Engineering
Unraveling Deep Receptor Profiles using High Dimensional Tool Sets - BD Biosciences 10:30am - 11:30am
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Over the last several decades the scientific community has greatly advanced our understanding of the immune system identifying hundreds of unique CD markers, cytokine and chemokine receptors as well as thousands of genes to characterize and isolate cellular subsets to better understand their function and role in the human condition. Simultaneously, technological advancements have provided researchers the ability to multiplex high numbers of these analytes at the single-cell level, increasing the size and velocity of data sets. Despite the increase in fluorescent channels available on high-parameter flow cytometers, the field still is hampered from being able to use large numbers of specificities. Recently with the introduction of antibody sequencing applications, the scaling of proteomics has now been initiated.Today we will highlight contributions from the scientific community paired with advances in BD high-parameter technology to simplify and streamline customer workflows including:
Session Moderators
Ben Johnson, MBA, Marketing Manager, BD Biosciences
Carrie Carruthers, Global Manager, Tradeshows, BD Biosciences
Unraveling Deep Receptor Profiles using High Dimensional Tool Sets
Robert Balderas, VP Biological Sciences, BD Biosciences
Overview
Over the last several decades the scientific community has greatly advanced our understanding of the immune system identifying hundreds of unique CD markers, cytokine and chemokine receptors as well as thousands of genes to characterize and isolate cellular subsets to better understand their function and role in the human condition. Simultaneously, technological advancements have provided researchers the ability to multiplex high numbers of these analytes at the single-cell level, increasing the size and velocity of data sets. Despite the increase in fluorescent channels available on high-parameter flow cytometers, the field still is hampered from being able to use large numbers of specificities. Recently with the introduction of antibody sequencing applications, the scaling of proteomics has now been initiated.Today we will highlight contributions from the scientific community paired with advances in BD high-parameter technology to simplify and streamline customer workflows including:
- Complementarity of the high-dimensional BD FACSymphony™A5 Cell Analyzer and BD FACSymphony™ S6 Cell Sorter platforms.
- Single-cell genomics via the BD Rhapsody™ Single-Cell Analysis System and BD® AbSeq Assay.
- Panel design and data analysis tools and datasets.
Agenda and Speaker
Session Moderators
Ben Johnson, MBA, Marketing Manager, BD Biosciences
Carrie Carruthers, Global Manager, Tradeshows, BD Biosciences
Unraveling Deep Receptor Profiles using High Dimensional Tool Sets
Robert Balderas, VP Biological Sciences, BD Biosciences
New Assays and Advances in the BD Rhapsody™ Platform: Driving Deeper Biological Insights in Single-Cell Multiomic Analyses - BD Biosciences 11:30am - 12:30pm
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High-throughput single-cell RNA-seq (scRNA-seq) has transformed our ability to study complex and heterogeneous cell populations, while platforms such as the BD Rhapsody™ Single-Cell Analysis System allow researchers to profile tens of thousands of single cells simultaneously. In addition to whole transcriptome and targeted mRNA profiling, recent advances such as BD® AbSeq Oligonucleotide-Conjugated Antibodies allow researchers to examine 100+ proteins alongside RNA in the same assay. With the release of BCR/TCR receptor profiling, the BD Rhapsody can now analyze an even wider repertoire of molecules from the same cell. Here we showcase multiomics approaches to identifying and studying cell populations, pairing gene expression, protein expression and immune-repertoire information from the same cells in a single assay. Together these solutions can be harnessed to answer complex and important biological questions.
BD, the BD Logo and Rhapsody are trademarks of Becton, Dickinson and Company or its affiliates. © 2020 BD. All rights reserved.
New Assays and Advances in the BD Rhapsody™ Platform: Driving Deeper Biological Insights in Single-Cell Multiomic Analyses
Maggie Nakamoto, PhD, Manager Single-Cell Multiomics Applications, BD Biosciences
Overview
High-throughput single-cell RNA-seq (scRNA-seq) has transformed our ability to study complex and heterogeneous cell populations, while platforms such as the BD Rhapsody™ Single-Cell Analysis System allow researchers to profile tens of thousands of single cells simultaneously. In addition to whole transcriptome and targeted mRNA profiling, recent advances such as BD® AbSeq Oligonucleotide-Conjugated Antibodies allow researchers to examine 100+ proteins alongside RNA in the same assay. With the release of BCR/TCR receptor profiling, the BD Rhapsody can now analyze an even wider repertoire of molecules from the same cell. Here we showcase multiomics approaches to identifying and studying cell populations, pairing gene expression, protein expression and immune-repertoire information from the same cells in a single assay. Together these solutions can be harnessed to answer complex and important biological questions.
BD, the BD Logo and Rhapsody are trademarks of Becton, Dickinson and Company or its affiliates. © 2020 BD. All rights reserved.
Agenda and Speaker
New Assays and Advances in the BD Rhapsody™ Platform: Driving Deeper Biological Insights in Single-Cell Multiomic Analyses
Maggie Nakamoto, PhD, Manager Single-Cell Multiomics Applications, BD Biosciences
Break 12:30pm - 1:00pm
View Exhibit Hall
The next session will begin at 1:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Overview
The next session will begin at 1:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Plenary Session 2: Subcellular Cytometry 1:00pm - 2:30pm
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Plenary 2 on Subcellular Cytometry will showcase new and emerging techniques for the study of cellular processes with a focus on intracellular function and dynamics. Three invited speakers will present on tools that enable measurements of extracellular vesicles, intracellular signaling, and sorting based on cell secretion. This session will thus highlight how cytometry and sorting technologies and methods can enable the characterization of cells at a molecular level.
Session Moderators
Andy Filby, PhD, Innovation Methodology and Application (IMA) Theme Lead and FCCF Director, Newcastle University
Jessica P. Houston, PhD, Associate Professor of Chemical & Materials Engineering, New Mexico State University
Multiplex Nanoscale Flow Cytometry to Image the Circulating Extracellular Vesicle Biome in Patients with Pancreatic, Breast, Lung, and Prostate Cancer versus Screen Negative Controls
Terry Morgan, PhD, Professor of Pathology, Obstetrics & Gynecology, and Biomedical Engineering, School of Medicine, Oregon Health & Science University
Connecting Life and Death: The BCL-2 Family Coordinates Mitochondrial Network Dynamics and Apoptosis
Vivian Gama, PhD, Assistant Professor, Department of Cell and Developmental Biology, Vanderbilt Center for Stem Cell Biology, Vanderbilt Brain Institute, Vanderbilt University
Fluorescence Activated Cell Sorting Based on Secreted Products Using Lab on a Particle Technology
Joseph de Rutte, MA/MS, PhD Candidate, Bioengineering, University of California, Los Angeles, Co-Founder and President, Partillion Bioscience
Overview
Plenary 2 on Subcellular Cytometry will showcase new and emerging techniques for the study of cellular processes with a focus on intracellular function and dynamics. Three invited speakers will present on tools that enable measurements of extracellular vesicles, intracellular signaling, and sorting based on cell secretion. This session will thus highlight how cytometry and sorting technologies and methods can enable the characterization of cells at a molecular level.
Agenda and Speakers
Session Moderators
Andy Filby, PhD, Innovation Methodology and Application (IMA) Theme Lead and FCCF Director, Newcastle University
Jessica P. Houston, PhD, Associate Professor of Chemical & Materials Engineering, New Mexico State University
Multiplex Nanoscale Flow Cytometry to Image the Circulating Extracellular Vesicle Biome in Patients with Pancreatic, Breast, Lung, and Prostate Cancer versus Screen Negative Controls
Terry Morgan, PhD, Professor of Pathology, Obstetrics & Gynecology, and Biomedical Engineering, School of Medicine, Oregon Health & Science University
Connecting Life and Death: The BCL-2 Family Coordinates Mitochondrial Network Dynamics and Apoptosis
Vivian Gama, PhD, Assistant Professor, Department of Cell and Developmental Biology, Vanderbilt Center for Stem Cell Biology, Vanderbilt Brain Institute, Vanderbilt University
Fluorescence Activated Cell Sorting Based on Secreted Products Using Lab on a Particle Technology
Joseph de Rutte, MA/MS, PhD Candidate, Bioengineering, University of California, Los Angeles, Co-Founder and President, Partillion Bioscience
Veritas se Revēlet: Computational Population Discovery and Sorting for Functional and Multi-Omics Analysis - FlowJo 1:00pm - 2:00pm
View Session
As the number of dimensions being interrogated rises, thorough identification and characterization of populations using traditional manual gating analysis becomes more challenging. Manual gating requires subjective selection of features and declaration of population membership using 1 or 2 dimensional plots, an approach that not only requires previous knowledge of the phenotypic expression patterns of populations being identified, but also invariably applies operator bias when creating and drawing gates. Computational methods for clustering attempt to overcome these limitations by classifying events into populations defined by all dimensions simultaneously as a region of local density within marker space, acting more objectively, and potentially identifying novel populations that would be ignored or missed with manual gating. But how to get started employing such methods? Which clustering and dimensionality reduction algorithms are the best ones to use? How do you compare many samples, identify cluster phenotypes and visualize the populations resulting from a clustering algorithm? And perhaps most important, how can you sort an algorithm-derived population for downstream functional and single cell multi-omics studies? Join us as we address these questions and discuss how to employ the newest suite of tools in FlowJo, which enable computational discovery and sorting.
Session Moderator
John Quinn, PhD, Director, Science and Product Development at BD Bioinformatics
Veritas se Revēlet: Computational Population Discovery and Sorting for Functional and Multi-Omics Analysis
Timothy Quinn Crawford, PhD, Field Application Scientist, Informatics, BD Life Sciences
Overview
As the number of dimensions being interrogated rises, thorough identification and characterization of populations using traditional manual gating analysis becomes more challenging. Manual gating requires subjective selection of features and declaration of population membership using 1 or 2 dimensional plots, an approach that not only requires previous knowledge of the phenotypic expression patterns of populations being identified, but also invariably applies operator bias when creating and drawing gates. Computational methods for clustering attempt to overcome these limitations by classifying events into populations defined by all dimensions simultaneously as a region of local density within marker space, acting more objectively, and potentially identifying novel populations that would be ignored or missed with manual gating. But how to get started employing such methods? Which clustering and dimensionality reduction algorithms are the best ones to use? How do you compare many samples, identify cluster phenotypes and visualize the populations resulting from a clustering algorithm? And perhaps most important, how can you sort an algorithm-derived population for downstream functional and single cell multi-omics studies? Join us as we address these questions and discuss how to employ the newest suite of tools in FlowJo, which enable computational discovery and sorting.
Agenda and Speaker
Session Moderator
John Quinn, PhD, Director, Science and Product Development at BD Bioinformatics
Veritas se Revēlet: Computational Population Discovery and Sorting for Functional and Multi-Omics Analysis
Timothy Quinn Crawford, PhD, Field Application Scientist, Informatics, BD Life Sciences
Break 2:30pm - 3:00pm
View Exhibit Hall
The next session will begin at 3:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Overview
The next session will begin at 3:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Panel Design Considerations for High Parameter Spectral Flow Cytometry - Sony 3:00pm - 4:00pm
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Spectral flow cytometers introduced by Sony enable the use of a wider variety of fluorochrome combinations than previously possible with traditional non-spectral systems. The ID7000™ spectral analyzer is the newest addition to the Sony portfolio. In this tutorial, we will highlight some aspects of spectral cell analysis based on examples from the new ID7000 spectral cell analyzer. We will explore the key considerations when designing multicolor panels for use on the new spectral cell analyzer and review the available fluorochromes with respect to their spectral profiles and highlight how similarities and differences between them affect unmixing and data display. We will also share ways to optimize panel design and gain efficiency in the use of controls to ensure the highest data quality when using spectral flow cytometry.
Panel Design Considerations for High Parameter Spectral Flow Cytometry
Jerry Barnhart, Field Application Specialist, Sony Biotechnology
Overview
Spectral flow cytometers introduced by Sony enable the use of a wider variety of fluorochrome combinations than previously possible with traditional non-spectral systems. The ID7000™ spectral analyzer is the newest addition to the Sony portfolio. In this tutorial, we will highlight some aspects of spectral cell analysis based on examples from the new ID7000 spectral cell analyzer. We will explore the key considerations when designing multicolor panels for use on the new spectral cell analyzer and review the available fluorochromes with respect to their spectral profiles and highlight how similarities and differences between them affect unmixing and data display. We will also share ways to optimize panel design and gain efficiency in the use of controls to ensure the highest data quality when using spectral flow cytometry.
Agenda and Speaker
Panel Design Considerations for High Parameter Spectral Flow Cytometry
Jerry Barnhart, Field Application Specialist, Sony Biotechnology
Special Plenary Session 3: Biomarkers in Drug Development 3:00pm - 5:00pm
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The importance of biomarkers in decision-making during drug development continues to increase. Cellular biomarkers in particular, have become critical components for novel immune therapies in oncology, cell and gene therapies, infectious diseases and vaccine development. In this session, three speakers working directly in area of drug development will discuss the impact of biomarkers. Meena Subramanyam, Vice President, Global Project Leader, Takeda will talk about the overall strategy of implementing biomarkers in the drug development process. Thomas Kleen, Chief Scientific Officer, Immodulon Therapeutics will discuss cellular biomarkers for novel cancer immunotherapies. His presentation will address critical technologies for cellular analysis, the importance of rigorous methods, and strategic collaborations between industry and academia. Meina Liang, Senior Director, Global Head of Integrated Bioanalysis, MedImmune/AstraZeneca will talk about how specific flow cytometric assay such as RO impacted decision making.
Session Moderators
Virginia Litwin, PhD, Strategic Advisor, Caprion Biosciences
Cherie Green, Senior Scientific Manager, Genentech
R[E]volution in Biomarker Utilization in Therapeutic Drug Development
Meena Subramanyam, PhD, Vice-President & Global Project Leader, Takeda Pharmaceutical Co.
Novel Immunomodulators and their Combinations Require a Broad, Adaptive Clinical Biomarker Strategy
Thomas Kleen, PhD, Chief Scientific Officer, Immodulon Therapeutics
Flow Cytometry-based Biomarker Assays for Decision Making in Drug Development
Meina Liang, PhD, Senior Director, Global Head of Integrated Bioanalysis, AstraZeneca
Overview
The importance of biomarkers in decision-making during drug development continues to increase. Cellular biomarkers in particular, have become critical components for novel immune therapies in oncology, cell and gene therapies, infectious diseases and vaccine development. In this session, three speakers working directly in area of drug development will discuss the impact of biomarkers. Meena Subramanyam, Vice President, Global Project Leader, Takeda will talk about the overall strategy of implementing biomarkers in the drug development process. Thomas Kleen, Chief Scientific Officer, Immodulon Therapeutics will discuss cellular biomarkers for novel cancer immunotherapies. His presentation will address critical technologies for cellular analysis, the importance of rigorous methods, and strategic collaborations between industry and academia. Meina Liang, Senior Director, Global Head of Integrated Bioanalysis, MedImmune/AstraZeneca will talk about how specific flow cytometric assay such as RO impacted decision making.
Agenda and Speakers
Session Moderators
Virginia Litwin, PhD, Strategic Advisor, Caprion Biosciences
Cherie Green, Senior Scientific Manager, Genentech
R[E]volution in Biomarker Utilization in Therapeutic Drug Development
Meena Subramanyam, PhD, Vice-President & Global Project Leader, Takeda Pharmaceutical Co.
Novel Immunomodulators and their Combinations Require a Broad, Adaptive Clinical Biomarker Strategy
Thomas Kleen, PhD, Chief Scientific Officer, Immodulon Therapeutics
Flow Cytometry-based Biomarker Assays for Decision Making in Drug Development
Meina Liang, PhD, Senior Director, Global Head of Integrated Bioanalysis, AstraZeneca
A Complete Immune Monitoring Solution with CyTOF; Ideal for Pandemics and Beyond - Fluidigm Corporation 4:00pm - 5:00pm
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Immune monitoring studies, especially in the age of COVID-19 disease, require cytometry assays that:
Session Moderator
Theresa Royer, Fluidigm Corporation
A Complete Immune Monitoring Solution with CyTOF; Ideal for Pandemics and Beyond
Frederik De Smet, PhD, Assistant Professor, University of Leuven, Belgium
Noah Saederup, PhD, Senior Product Manager, Maxpar Reagents, Fluidigm
Introducing Flow Conductor, a sample preparation system for flow and mass cytometry
Ofir Goldberger, PhD, Director, Corporate Development, Fluidigm
Overview
Immune monitoring studies, especially in the age of COVID-19 disease, require cytometry assays that:
- are quick to implement with an easy workflow;
- have fixation embedded early in sample processing;
- are high parameter to ensure the most information possible per cell;
- have a streamlined, reproducible and unbiased data analysis pipeline; and
- have proven reproducibility when performed at distant sites.
Agenda and Speakers
Session Moderator
Theresa Royer, Fluidigm Corporation
A Complete Immune Monitoring Solution with CyTOF; Ideal for Pandemics and Beyond
Frederik De Smet, PhD, Assistant Professor, University of Leuven, Belgium
Noah Saederup, PhD, Senior Product Manager, Maxpar Reagents, Fluidigm
Introducing Flow Conductor, a sample preparation system for flow and mass cytometry
Ofir Goldberger, PhD, Director, Corporate Development, Fluidigm
Break 5:00pm - 5:30pm
View Exhibit Hall
The next session will begin at 5:30pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Overview
The next session will begin at 5:30pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Special Plenary Session 4: SRL LIVE 5:30pm - 6:30pm
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Shared Resource Laboratories (SRLs) occupy a unique niche in scientific research by facilitating technology integration and advancement as well as promoting collaboration across disciplines and platforms. In this session, Robert Salomon will share his experience of building and developing multidisciplinary teams to support cytometry applications both in Australia and internationally. Our second speaker, Dr. Nicole Poulton will discuss the application of technology advancements in aquatic cytometry.
Session Moderators
Jessica Back, PhD, Deputy Director, Microscopy, Imaging, and Cytometry Resources Core, Karmanos Cancer Institute, Wayne State University
Anna Belkina, MD, PhD, Assistant Professor of Pathology and Laboratory Medicine, Associate Director, Flow Cytometry Core Facility, Boston University School of Medicine
New Models for the Cytometry Shared Resource Laboratory - Building Multidisciplinary Teams to Advance Biomedical Science and Clinical Outcomes
Robert Salomon, MSc, Operations and Technology Manager, ACRF Child Cancer Liquid Biopsy Program (ACRF CCLBP), Children's Cancer Institute
Technology Breakthroughs in Aquatic Cytometry: A New Understanding of the Ocean Microbiome
Nicole Poulton, PhD, Director, Center for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences
Overview
Shared Resource Laboratories (SRLs) occupy a unique niche in scientific research by facilitating technology integration and advancement as well as promoting collaboration across disciplines and platforms. In this session, Robert Salomon will share his experience of building and developing multidisciplinary teams to support cytometry applications both in Australia and internationally. Our second speaker, Dr. Nicole Poulton will discuss the application of technology advancements in aquatic cytometry.
Agenda and Speakers
Session Moderators
Jessica Back, PhD, Deputy Director, Microscopy, Imaging, and Cytometry Resources Core, Karmanos Cancer Institute, Wayne State University
Anna Belkina, MD, PhD, Assistant Professor of Pathology and Laboratory Medicine, Associate Director, Flow Cytometry Core Facility, Boston University School of Medicine
New Models for the Cytometry Shared Resource Laboratory - Building Multidisciplinary Teams to Advance Biomedical Science and Clinical Outcomes
Robert Salomon, MSc, Operations and Technology Manager, ACRF Child Cancer Liquid Biopsy Program (ACRF CCLBP), Children's Cancer Institute
Technology Breakthroughs in Aquatic Cytometry: A New Understanding of the Ocean Microbiome
Nicole Poulton, PhD, Director, Center for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences
From Data to Insights: Friendly Analysis of Cytometry Data Using Astrolabe - Astrolabe 5:30pm - 6:30pm
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The Astrolabe Cytometry Platform is a fast, reliable tool for the analysis of high-complexity cytometry data. Our platform provides efficient, reproducible results in 1-2 hours. Pinpoint biological insights with our intuitive interface and access paper-ready data visualizations with a mouse click. Join Dr. El-ad David Amir, Astrlabe's CEO, to learn about our cloud-based, peer-reviewed solution and how they can help you unlock insights from your data.
From Data to Insights: Friendly Analysis of Cytometry Data Using Astrolabe
El-ad David Amir, PhD, Chief Executive Officer, Astrolabe Diagnostics, Inc.
Overview
The Astrolabe Cytometry Platform is a fast, reliable tool for the analysis of high-complexity cytometry data. Our platform provides efficient, reproducible results in 1-2 hours. Pinpoint biological insights with our intuitive interface and access paper-ready data visualizations with a mouse click. Join Dr. El-ad David Amir, Astrlabe's CEO, to learn about our cloud-based, peer-reviewed solution and how they can help you unlock insights from your data.
Agenda and Speaker
From Data to Insights: Friendly Analysis of Cytometry Data Using Astrolabe
El-ad David Amir, PhD, Chief Executive Officer, Astrolabe Diagnostics, Inc.
Break 6:30pm - 7:00pm
View Exhibit Hall
The next session will begin at 7:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Overview
The next session will begin at 7:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!
Swimming with Elephants: Solving our Fluorescence Infrastructure Problem - Phitonex 7:00pm - 8:00pm
View Session
Fluorescence is the critical infrastructure on which our experiments, from imaging to flow cytometry and sorting, travel. Traditional dyes and the consequent hardware response are holding us back from easier experimental design, data analysis, high purity sorting, and multi-omic measurement. In this tutorial, we will describe our progress in massively expanding the number of questions per cell in flow cytometry leveraging our labels -- the largest set of new fluorophores than have been released in the past 20 years. Additionally, we will describe fluorescence for biology, and how to design fluorescent labels with specific, controllable requirements for excitation, emission, and brightness. We will show our work in producing conjugated antibodies and pushing the envelope across all instruments - from clean experiments on 12 detector instruments to well beyond 40 colors. We also share our customers’ success stories and share why one customer described our products as “The best addition to flow cytometry in the last 10 years.”We will also take on and reveal solutions to several elephant-in-the-room problems in our field: (1) the stability and performance of tandem dyes, (2) the heuristics of panel design, (3) the divide between genomic and flow cytometry, (4) lot to lot inconsistency, and (5) innovation in critical infrastructure.
Swimming with Elephants: Solving our Fluorescence Infrastructure Problem
Mike Stadnisky, PhD, CEO, Phitonex
Seddon Thomas, PhD, Research Application Scientist, Phitonex
Overview
Fluorescence is the critical infrastructure on which our experiments, from imaging to flow cytometry and sorting, travel. Traditional dyes and the consequent hardware response are holding us back from easier experimental design, data analysis, high purity sorting, and multi-omic measurement. In this tutorial, we will describe our progress in massively expanding the number of questions per cell in flow cytometry leveraging our labels -- the largest set of new fluorophores than have been released in the past 20 years. Additionally, we will describe fluorescence for biology, and how to design fluorescent labels with specific, controllable requirements for excitation, emission, and brightness. We will show our work in producing conjugated antibodies and pushing the envelope across all instruments - from clean experiments on 12 detector instruments to well beyond 40 colors. We also share our customers’ success stories and share why one customer described our products as “The best addition to flow cytometry in the last 10 years.”We will also take on and reveal solutions to several elephant-in-the-room problems in our field: (1) the stability and performance of tandem dyes, (2) the heuristics of panel design, (3) the divide between genomic and flow cytometry, (4) lot to lot inconsistency, and (5) innovation in critical infrastructure.
Agenda and Speakers
Swimming with Elephants: Solving our Fluorescence Infrastructure Problem
Mike Stadnisky, PhD, CEO, Phitonex
Seddon Thomas, PhD, Research Application Scientist, Phitonex
Plenary Session 3: Extracellular & Oceanic Cytometry 7:00pm - 8:30pm
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The session focuses on the small but in wider contexts. From a background of research on the co-evolution of microbe and virus (phage) in environmental populations, as well as the impact of marine phages on microbe-mediated biogeochemistry, global sampling approaches are revealing are revealing new aspects of oceanic ecosystems. The session features studies on extracellular vesicles (EVs) - the secretion of which from cells promotes aggressive, invasive behavior and facilitates tumor growth and metastasis, while simultaneously presenting a challenge for both their characterisation and exploitation by cytometry.
Session Moderators
Thomas Ashhurst, PhD, High-Dimensional Cytometry Specialist, University of Sydney
Joshua Welsh, PhD, Postdoctoral Fellow (CRTA), Laboratory of Pathology, Center for Cancer Research, National Cancer Institute
Emergent Ecosystem Properties Revealed by Global Sampling and the Tara Oceans Cytometric and Imaging Pipeline
Matthew Sullivan, PhD, Professor, Departments of Microbiology and Environmental and Geodetic Engineering, Ohio State University
Rigor and Standardization in Extracellular Vesicle Characterization
Kenneth W. Witwer, PhD, Associate Professor of Molecular and Comparative Pathobiology, Neurology, and Cellular & Molecular Medicine, Johns Hopkins University School of Medicine
Role of Exosomes in Cancer Aggressiveness
Alissa Weaver, MD, PhD, Cornelius Vanderbilt Professor of Cell and Developmental Biology, Vanderbilt University School of Medicine
Overview
The session focuses on the small but in wider contexts. From a background of research on the co-evolution of microbe and virus (phage) in environmental populations, as well as the impact of marine phages on microbe-mediated biogeochemistry, global sampling approaches are revealing are revealing new aspects of oceanic ecosystems. The session features studies on extracellular vesicles (EVs) - the secretion of which from cells promotes aggressive, invasive behavior and facilitates tumor growth and metastasis, while simultaneously presenting a challenge for both their characterisation and exploitation by cytometry.
Agenda and Speakers
Session Moderators
Thomas Ashhurst, PhD, High-Dimensional Cytometry Specialist, University of Sydney
Joshua Welsh, PhD, Postdoctoral Fellow (CRTA), Laboratory of Pathology, Center for Cancer Research, National Cancer Institute
Emergent Ecosystem Properties Revealed by Global Sampling and the Tara Oceans Cytometric and Imaging Pipeline
Matthew Sullivan, PhD, Professor, Departments of Microbiology and Environmental and Geodetic Engineering, Ohio State University
Rigor and Standardization in Extracellular Vesicle Characterization
Kenneth W. Witwer, PhD, Associate Professor of Molecular and Comparative Pathobiology, Neurology, and Cellular & Molecular Medicine, Johns Hopkins University School of Medicine
Role of Exosomes in Cancer Aggressiveness
Alissa Weaver, MD, PhD, Cornelius Vanderbilt Professor of Cell and Developmental Biology, Vanderbilt University School of Medicine
Late Night Cytometry: A Reflection on CYTO Virtual 2020 and What’s Next for Cytometry 8:30pm - 9:20pm
View Session
This networking session will consist of an informal discussion revolving around all things cytometry, with a special focus on reviewing presentations from CYTO Virtual 2020. Cytometry leaders from the region will discuss highlights from the meeting and provide an insight into where they think cytometry is going in the future. Audience participation will be encouraged through the use of polling. This session has purposely been scheduled in a time slot that is accessible to ISAC members in the Asia/Pacific region. Learning Objectives:
Session Moderator
Robert Salomon, MSc, Operations and Technology Manager ACRF Child Cancer Liquid Biopsy Program (ACRF CCLBP) Children's' Cancer Institute, Former ISAC SRL Emerging Leader
Panelists
Helen McGuire, PhD, Research Fellow, University of Sydney, Australia, ISAC Marylou Ingram Scholar
Lihong Wang, PhD, Bren Professor of Medical Engineering and Electrical Engineering, California Institute of Technology
Qianjun Zhang, MS, Staff Application Scientist, Beckman Coulter Life Sciences, Emeritus ISAC Marylou Ingram Scholar
Nao Nitta, PhD, Founder & President, CYBO, ISAC Marylou Ingram Scholar
Paul Hutchinson, PhD, Head of Flow Cytometry Facility, Life Sciences Institute of the National University of Singapore
Overview
This networking session will consist of an informal discussion revolving around all things cytometry, with a special focus on reviewing presentations from CYTO Virtual 2020. Cytometry leaders from the region will discuss highlights from the meeting and provide an insight into where they think cytometry is going in the future. Audience participation will be encouraged through the use of polling. This session has purposely been scheduled in a time slot that is accessible to ISAC members in the Asia/Pacific region. Learning Objectives:
- To provide a wrap-up of the current state of cytometry as illustrated by a discussion of the presentations at CYTO Virtual 2020
- To provide a perspective for what cytometry may look like in the future
- Gain a consensus on what opportunities and challenges cytometry faces in the coming years
Agenda and Speakers
Session Moderator
Robert Salomon, MSc, Operations and Technology Manager ACRF Child Cancer Liquid Biopsy Program (ACRF CCLBP) Children's' Cancer Institute, Former ISAC SRL Emerging Leader
Panelists
Helen McGuire, PhD, Research Fellow, University of Sydney, Australia, ISAC Marylou Ingram Scholar
Lihong Wang, PhD, Bren Professor of Medical Engineering and Electrical Engineering, California Institute of Technology
Qianjun Zhang, MS, Staff Application Scientist, Beckman Coulter Life Sciences, Emeritus ISAC Marylou Ingram Scholar
Nao Nitta, PhD, Founder & President, CYBO, ISAC Marylou Ingram Scholar
Paul Hutchinson, PhD, Head of Flow Cytometry Facility, Life Sciences Institute of the National University of Singapore
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