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  • Contains 3 Component(s), Includes Credits

    A CYTO U Webinar presented by Caroline E. Roe, MLI Keywords: High dimensional cytometry, Spectral flow, Mass cytometry

    About the Speaker

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    Caroline E. Roe, MLI
    Managing Director, Cancer and Immunology Core
    Vanderbilt University, Nashville, TN, USA

    Ms. Roe received her B.S. in Genetics, Cell Biology, and Development from the University of Minnesota-Twin Cities and Master of Laboratory Investigation from Vanderbilt University in Nashville, Tennessee, where she has managed the Mass Cytometry Center of Excellence since its inception in 2017. As director of the Cancer and Immunology Core at Vanderbilt, her focus is on increasing both the standardization and accessibility of cutting-edge cytometry techniques for application across a broad range of basic and translational research projects.  

    Webinar Summary
    This webinar aims to give an experience-driven overview of considerations for choosing the appropriate high dimensional flow cytometry technology for a project as well as guidance for those who have experience in one technology and are transitioning to the other.

    Learning Objectives
    Attendees will leave the webinar with a strong foundation in high dimensional cytometry principles and applications, as well as sufficient working knowledge to select the appropriate technology for their study.  

    Who Should Attend: Users who are interested in high-dimensional cytometry of any kind. 

    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    A CYTO U Webinar presented by Kylie Price Keywords: Shared Resource Laboratories, team science, health research culture, equality and diversity

    About the Speaker

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    Kylie Price
    Chief Technology Officer
    New Castle University
    Malaghan Institute of Medical Research

    Kylie Price is the Chief Technology Officer at the Malaghan Institute of Medical Research with 20 years’ experience providing strategic, scientific, and operational direction in multidisciplinary environments.  Kylie has strong stakeholder management and engagement skills and has attracted more than $35m of philanthropic funding over the past 12 years, supporting the creation of a distinguished centre of research excellence, the Hugh Green Cytometry Centre (HGCC).  Kylie leads a team of twelve highly skilled staff scientists in providing support to over 90 scientists at the Malaghan Institute.  The HGCC provides access to cutting-edge technology platforms (including full spectrum flow cytometry, histology, bioimaging, data science, molecular biology and mRNA for pre-clinical vaccine development), and she advises multiple research groups and companies both nationally and abroad. Kylie also has a strong leadership track-record, organizing high-profile networking events, such as CYTOAsia Singapore 2017, and directing international organizations, such as the Australasian Cytometry Society of which she is former President (2015-2016).  Kylie is a two-time NZ Woman of Influence Awards (2014 and 2018) finalist and finalist of the 2021 NZ High Tech Awards.  She was the first New Zealander elected to the International Society for Advancement of Cytometry (ISAC) Council (2018-2020) and is currently serving as Chair of the Governance Committee and Secretary of ISAC (2020-2024).  In April 2023 Kylie was selected to join the NZ OnBoard Program and is currently a board observer for Orbis Diagnostics.

    Webinar Summary

    This webinar will cover an overview of Kylie’s career path and provide examples of how she promotes equity, employee engagement and participation within her team.  She will cover the SRL Career Progression Framework she developed with others at the Malaghan Institute of Medical Research and discuss how she encourages the appropriate recognition of SRLs and SRL staff scientists in publication. There will be a Q+A session and we hope for lots of questions and engagement.

    Learning Objectives

    1. New ideas for how to increase SRL staff engagement and participation 
    2. A guideline to follow for the appropriate recognition of SRLs and SRL staff scientists in publication
    3. An example of a non-traditional career path to Chief Technology Officer
    4. Strategies for promoting equity in the workplace

    Who Should Attend: Scientists who manage teams (PIs, researchers, SRL managers), SRL staff scientists, women in STEM, scientists interested in topics related to equity and inclusion.

    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    Use of Spectral Flow Cytometry in Clinical Trials By Veronica Nash and Tania Nevers

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    Veronica Nash, PhD
    US Regional Head of Flow Cytometry
    Cerba Research

    Veronica earned her B.Sc and M.Sc in Medical Biotechnologies from “Tor Vergata” and from “Sapienza” University, respectively, in Rome, Italy. She continued her training at Ghent University, Belgium where she received her PhD in Medical Sciences. After her PhD, she joined Icahn School of Medicine at Mount Sinai in NYC where she leveraged her knowledge in virology and immunology with her Flow Cytometry expertise to identify host cofactors in the immune cell which are involved in HIV and HTLV infection. Afterwards, she moved to Indiana University in Bloomington, IN, and as a staff scientist she optimized a Flow Cytometry method to identify protein mutants to be used as a target in precision medicine for Cystic Fibrosis. Veronica’s career in industry started at Wuxi Apptec where she developed Flow Cytometry assays for QC of drug therapy products, such as CAR-T cells and TILs. Then she joined Labcorp Drug development (Covance) as a staff scientist where she focused on Flow cytometry assay validation, data review and instrument implementation. Currently, as the US Regional Head of Flow Cytometry department at Cerba Research, she is the scientific lead for high-parameters Flow Cytometry; in the last year, she led a global team through the implementation and harmonization of Cytek Aurora instruments across multiple sites. Veronica is also an active member of ISAC and ICCS; currently she is the leader of a working group which aims at promoting CLSI H62 guidelines education within the Flow Cytometry community.

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    Tania Nevers
    Senior Principal Scientist
    Bristol Myers and Squibb

    Tania earned her B.Sc and M.Sc in Biology from St John’s University, Queens NY before receiving her PhD in Biomedical Sciences from Brown University in Providence, RI. Following her Ph.D., Tania pursued a postdoctoral training at Tuft’s University where her work combined areas of immunology, vascular biology and cardiac physiology to study several aspects of adaptive immunity in diverse inflammatory settings. She then moved on to a Contract Research Organization, Flowmetric Inc, where she served as a  Scientist and then Project Manager. Her primary responsibility was to design, review and execute complex flow cytometry experiments in both preclinical and clinical studies for client sponsored projects. She then transitioned to the large Pharmaceutical Industry where she currently resides as a Senior Principal Scientist at Bristol Myers and Squibb. She primarily works on high order assay development, validation and implementation, and is the subject matter expert for Cytek Aurora implementation across BMS, including integration and harmonization activities.   

    When not at work, Tania enjoys spending quality time with family as well as listening to music, dancing and gardening. 

    About the webinar:

    The use of Spectral Flow Cytometry allows for a deeper characterization of immune subsets (immune profiling) than conventional flow cytometry and is more valuable with limited patient samples in biomarker discovery in drug development. This webinar describes how to implement and standardize Cytek Aurora instruments and how to design, develop, and validate 30+ parameters panels that can be successfully utilized in clinical trials.

    Learning Objectives:

    • Learn how to implement and standardize Cytek Aurora instruments
    • Understand how to validate an assay for 30+ colors on a spectral instrument to use in clinical trials
    • Learn solutions on challenges such as reference controls, unmixing strategies and experimental sept up when implementing a high-parameter assay for global clinical trials 

    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    ISAC Biosafety Committee Panel Discussion – Topics and Challenges in Biosafety

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    Evan Jellison
    Associate Professor & Director of Flow Cytometry
    UCONN School of Medicine

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    Kristen Reifel
    Vaccine Research Center
    NIAID/NIH

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    Benjamin Fontes, MPH, CBSP
    Biosafety Officer
    Yale University Environmental Health & Safety

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    Avrill Aspland
    Operations Coordinator
    Sydney Cytometry

    About the webinar:

    This webinar will be an open discussion with members of the ISAC Biosafety Committee to share experiences of biosafety and how we work to seek continual improvements in our shared resource laboratories.  We will discuss the strategies we use to identify priorities, initiate improvements, and embed new ways of doing things.  We will also respond to biosafety-related questions submitted through a pre-webinar survey and to questions and comments submitted in real time through a live Q&A. Please complete the pre-webinar survey sent to your email!

    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    The Journey of Achieving High Standards in Multiparametric Flow Cytometry Data By Maria Jaimes Keywords: High-Quality Data, Standardization, Careers in Flow Cytometry

    The Journey of Achieving High Standards in Multiparametric Flow Cytometry Data By Maria Jaimes

    About the Presenter

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    Maria Jaimes
    VP Scientific Commercialization
    Cytek Biosciences 

    Dr. Maria Jaimes earned her MD degree at the Universidad Javeriana in Colombia (South America). Dr. Jaimes completed her postdoctoral training at Stanford University in the department of Microbiology and Immunology. During her postdoc, she worked at characterizing the immune responses to both rotavirus and influenza viruses after natural infection and immunization. In 2005, Dr. Jaimes joined BD Biosciences.  While at BD, Maria worked in different aspects of quality assurance and standardization of flow cytometry assays. Since 2015, Maria has been working at Cytek Biosciences and is part of the R&D team who developed the Aurora Full Spectrum Cytometer. Dr. Jaimes has overseen the instrument characterization, verification and development of multicolor applications. Besides her responsibilities within the R&D team, Dr. Jaimes leads the Technical Applications Support team worldwide

    Webinar Summary

    Dr. Jaimes will discuss her career in flow cytometry with emphasis on her work around standardization of flow cytometry assays and development of multicolor assays.  Dr. Jaimes will also discuss her journey of applying her knowledge in multicolor flow cytometry into the development of a new technology while working at a start-up, with the motivation of contributing to the generation of high-quality data. Maria will discuss the challenges faced by those dedicated to disseminating best practices in flow cytometry and the role that both academia and industry should play at enforcing those.


    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    A CYTO U Webinar presented by Andrew Filby, PhD Keywords: Shared Resource Laboratories, team science, health research culture, equality and diversity

    About the Speaker

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    Andrew Filby, PhD
    Director of the Newcastle University Flow Cytometry and Single Cell Technologies Shared Resource Laboratory
    New Castle University

    Dr. Filby is currently the director of the Newcastle University Flow Cytometry and Single Cell Technologies Shared Resource Laboratory. He also leads the “Innovation, Methodology and Application” (IMA) crosscutting research Theme in the Faculty of Medical Sciences (FMS), where he oversees about 350 members from different technology and methodology backgrounds that includes both academic and technical job families. Dr. Filby is also a director of the Newcastle University Centre of Research Excellence (NUCoRE) in Biomedical Engineering. Dr. Filby and his team have an active program of research that includes the development of novel methods for single cell analysis and evaluating new technologies. He publishes in high-impact journals such as Science and Nature and sits on several funding panels for equipment grants. He recently won the Times Higher Education (THE) “Outstanding Technician of the Year” in 2021 for his work on breaking down barriers and establishing parity of esteem for non-academic researchers.

    Webinar Summary

    In this webinar Dr. Filby will discuss how the “Shared Resource Laboratory” (SRL) can be a catalyst for establishing a culture of team science, inclusivity, and health research culture. He will also discuss his role as lead of the “Innovation, Methodology and Application” (IM) cross-cutting research theme within the faculty of medical sciences at Newcastle University. Dr. Filby will also address the imbalance that sometimes exists between academic and technical job families and how he has worked via the “Innovation, Methodology and Innovation” (IMA) research Theme that he leads to try and overcome this.

    Learning Objectives

    • How the shared resource laboratory (SRL) can be a catalyst and “rally point” for team science and a healthy research culture.
    • How the right attitudes to research culture can create an inclusive and effective environment for success.
    • How technology and methodology expertise can drive discovery and impact.

    Who Should Attend

    • Anyone working in or interested in working in an SRL.
    • Anyone interested in or involved in team science.

    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    A Basic Introduction to the Science of Cell Sorting By Matthew Goff Cell Sorting, Flow Cytometry Basics

    A Basic Introduction to the Science of Cell Sorting By Matthew Goff

    About the Presenter

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    Matthew Goff
    Senior Product Manager, Flow Cytometry
    Beckman Coulter Life Science

    Matthew began his career in flow cytometry doing graduate research at Virginia Tech followed by his role as a core lab manager at Eastern Virginia Medical School. After leaving EVMS for industry, Matthew stayed close to flow cytometry supporting researchers and industrial scientists in flow cytometry with reagents, software, and hardware products. In 2021 he became a commercial product manager at Beckman Coulter Life Sciences where his work is focused on sustaining the CytoFLEX Platform and launching new products. 

    Webinar Summary
    This webinar introduces scientists with an interest in learning more about flow cytometry an introduction to the mechanics of cell sorting, best practices when sorting cells and contemporary discussion topic associated with cell sorting.


    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    Should CYTO Women Go with the Flow? By Virginia Litwin Keywords: Mentorship, Sponsorship, Bystander Training, STEM, Women in Cytometry

    Should CYTO Women Go with the Flow? By Virginia Litwin

    About the Presenter

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    Virginia Litwin
    Scientist
    Charles River Labs

    Virginia Litwin is a thought-leader in validation and standardization for flow cytometry. Bringing “Cytometry from Bench-to-Bedside” has been the focus of her professional activities since 1999 when she started working in translational medicine at Bristol-Myers Squibb. 

    She co-founded the Flow Cytometry Community within the American Association of Pharmaceutical Scientists (AAPS). This group published the first papers on flow cytometry method and instrument validation. She was a counselor for the International Society for the Advancement of Cytometry (ISAC) and the International Clinical Cytometry Society (ICCS). She serves on the ICCS Advocacy Committee whose mission is to interface with regulatory agencies and has been an invited speaker at FDA/NIST on many occasions.

    Virginia is the chair of the Document Development Committee for the guidance document, CLSI H62- The Validation of Assays Performed by Flow Cytometry. She edited the book, Flow Cytometry in Drug Discovery and Development, as well as journal special issues: JIM- Flow Cytometry Biomarkers and Translational Medicine (2011); Cytometry Part B- Receptor Occupancy (2015); Cytometry Part B- Cytometry Advancing Next Generation Drug Development (2021).

    After obtaining a Ph.D. in Virology/Immunology from the University of Iowa, Virginia joined Lewis Lanier at DNAX as a post-doctoral fellow where she identified the KIR receptor, KIR3DL1 (CD158E1). She has held leadership roles in several contract research organizations. Currently she is a Research Scientist at Charles River Laboratories in Québec, Canada.

    Webinar Summary

    This webinar will review a wide variety of topics which impact the careers of CYTO Women. The importance of both being a Mentor and a Mentee will be reviewed. Case studies of how Sponsorship can impact an individual career and CYTO Women in general will be presented. Lastly, we will discuss how and when to change the things we cannot accept. Most of the webinar will be Q&A and hopefully a very lively discussion.


    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits

    Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox Keywords: Flow Cytometry, Cancer Research, Radiotherapy, Shared Resource Laboratory, Instrumentation

    Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox

    About the Presenter

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    Dagna Sheerar
    Flow Cytometry Director
    University of Wisconsin - Madison
    Carbone Cancer Center Flow Cytometry Laboratory

    Dagna Sheerar, SCYM(ASCP)CM, has been working in flow cytometry shared resource laboratories for over 20 years and is currently the director of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow).  UWFlow is a busy core with a staff of 7 full time flow cytometrists, including Dagna, with 2 core laboratory locations on campus.  Dagna’s areas of interest are core administration and management, rigor and reproducibility, and user education. Since becoming director, Dagna has successfully added, on average, one instrument per year to the Flow Lab through various grant programs, institutional purchases, or “crowd funding.” Dagna has been involved in the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) for almost as long as she has been working in cores, and she recently served as GLIIFCA president from 2020-2021 and currently serves on their Board of Directors. She is a member of ISAC and serves on the SRL Content Task Force. She serves as host, organizer, and instructor for the Annual Course in Cytometry, and recently completed the 45th Annual Course held at the University of Wisconsin in June of 2022. Dagna has presented at GLIIFCA, the annual CYTO Meeting, and the Association of Biomolecular Resource Facility (ABRF) annual meetings. Dagna also served as the Interim Director of Campus Research Cores at UW – Madison in 2020.  

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    Kathryn Fox
    Flow Lab Technical Manager
    University of Wisconsin
    Carbone Cancer Center Flow Cytometry Laboratory

    Kathryn Fox, SCYM(ASCP)CM, is currently the technical manager of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow). She has been working for the UW Carbone Cancer Center Flow Cytometry Core Laboratory since 2015. Her flow cytometry interests include digging around inside instruments, any application that involves staining DNA, and being surprised by interesting new projects brought forward by the core’s diverse user base. Kathryn graduated from Iowa State University with a BS in Biology and then from the University of Wisconsin – Madison with a PhD in Cancer Biology. She was first introduced to flow cytometry in graduate school, but her main focus at that time was fluorescence microscopy. After graduate school, she worked as an applications scientist in multiphoton microscopy. Since diving into flow cytometry, Kathryn has been an active participant in GLIIFCA and the Annual Course in Cytometry, and finally got to attend her first in-person CYTO in 2022. 

    Webinar Summary

    This seminar will focus on how a flow core can respond to the needs of researchers to analyze radiotherapy treated samples in real time via flow cytometry.  We will present our process for providing this service from the initial idea, partnering with our Small Animal Imaging and Radiotherapy Facility, working with our institutional Radiation Safety group to test various cleaning methods for removing radioactive particles from the instrument post-analysis, instrument modifications, working with the original equipment manufacturer to ensure compliance with our existing service contract and safety for the engineers servicing the equipment, and additional safety measures and training procedures developed specific to this service.


    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    A CYTO U Webinar presented Kewal Asosingh, PhD Keywords: Endothelial, flow cytometry, rare event detection

    About the Speaker

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    Kewal Asosingh, PhD
    Scientific Director
    Cleveland Clinic Lerner Research Institute

    Dr. Asosingh is a principal investigator and scientific director for Flow Cytometry at the Cleveland Clinic Lerner Research Institute. He is a past ISAC Scholar, Cytometry Part A Associate Editor, ISAC Flow Cytometry Content, and Education Committee member. His flow cytometric research is centered on lung vascular biology.

    Webinar Summary

    Endothelial cells are cells that reside in tissues and circulate in the bloodstream in small numbers. Immunophenotyping these cells has several challenges, like how to detect endothelial cells, how to gate them, and how to figure out if they're really endothelial. All of this will be covered in this webinar.

    Learning Objectives

    • Immunophenotype of murine and human circulating and organ-specific endothelial cells
    • Endothelial cell gating strategies and common pitfalls
    • The dos and don'ts of functional endothelial cell characterization

    Who Should Attend

    Flow cytometry SRL members and anyone interested in endothelial cell flow cytometry.

    CMLE Credit: 1.0

  • Contains 6 Product(s)

    Cytometry panels packaging.

    • The Road to a High-Resolution 40-Color Flow Cytometry Immunophenotyping Panel
    • Designing Panels for the Study of Hematopoietic Stem Cells
    • Panel Design - A Practical Guide for Successful Fluorescent Cytometry Panels from 10-40 Parameters
    • Predicting the Best Resolution and Sensitivity in Panel Development and Reducing Inter-instrument Variability in Flow Cytometry
    • 16-Color Panel to Measure Inhibitory Receptor Signatures from Multiple Human Immune Cell Subsets
    • Step-by-Step Multi-parameter Panel Design 
  • Contains 5 Product(s)

    An overview of fluorescence cytometry

    • Single Cell Analysis of Autofluorescence Lifetime Images 
    • Multiplexed Fluorescence Microscopy Reveals Heterogeneity among Stromal Cells in Mouse Bone Marrow Sections
    • Evaluating Spectral Cytometry for Immune Profiling in Viral Disease
    • The Essentials of Optics and Fluorescence Microscopy for Cell Biology Applications (2013 Advanced Data Analysis Pre-Congress Course)
  • Contains 3 Product(s)

    Flow cytometry and hematology package.

    • Designing Panels for the Study of Hematopoietic Stem Cells
    • Flow Cytometric Analysis of Endothelial Colony Forming Cells and Hematopoietic Progenitor Cells in Lung Vascular Disease by Kewal Asosingh and Imaging Flow Cytometry in the Study of Immune Cell Functions by Andrew Filby
    • Flow Cytometry Analysis of Human Hematopoietic Progenitors in Cardiovascular Disease
  • Contains 3 Component(s), Includes Credits Recorded On: 05/28/2020

    A CYTO U Webinar presented by Andrea Cossarizza, PhD Keywords: immunophenotyping, viral infection, SARS-CoV-2, high-dimensional cytometry

    About the Presenter

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    Andrea Cossarizza
    Full Professor of Pathology and Immunology
    University of Modena and Reggio Emilia

    Andrea Cossarizza is a full professor of pathology and immunology, vice president of the Faculty of Medicine at the University of Modena and Reggio Emilia, and former president of the International Society for Advancement of Cytometry (ISAC). He has been studying the molecular and cellular basis of immune system-based diseases for 35 years. He is currently at the forefront of the fight against COVID-19 and has provided the first contributions regarding the importance of cytometry in understanding the immune response to SARS-CoV-2. As of May 2020, he has published 332 papers in peer-reviewed journals, has an H index of 79, and received over 33,000 citations.

    Webinar Summary

    The immune system is heavily involved in the pathogenesis of COVID-19 and its activation (which includes the so-called cytokine storm) and is the main force that drives the course of the infection. This webinar will present the most recent data on main changes that occur among different lymphocyte populations, along with functional analysis of T cells, and discuss current and possible therapeutic approaches.

    Learning Objectives

    • Describe the main changes that occur in the immune system during different phases of the infection with SARS-CoV-2, the virus that causes COVID-19.
    • Identify cytometric techniques that have been used to better understand changes that occur in the immune system during the infection and its recovery.

    Who Should Attend

    Researchers, clinicians, laboratory managers, and personnel involved in the fight against SARS-CoV-2 who want to know the latest discoveries on the role of the immune system during COVID-19.

    CMLE Credit: 1.0

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  • Contains 3 Product(s)

    Data analysis series package.

    • Data Analysis Rigor and Reproducibility, Part 1: Experimental Design
    • Data Analysis Rigor and Reproducibility, Part 2: Analysis Tools
    • Data Analysis Rigor and Reproducibility, Part 3: Publishing Flow Cytometry Data
  • Contains 3 Component(s), Includes Credits Recorded On: 10/22/2019

    A CYTO U Webinar presented by Aja Rieger, PhD & Andrew Filby, PhD Keywords: publication, visualisation, guidelines, MIFlowCyt, Flow Cytometry, Mass Cytometry, Genomic Cytometry

    About the Presenters

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    Aja Rieger, PhD
    Flow Core Manager
    University of Alberta

    Dr. Rieger graduated from the University of Alberta with a BSc honors in immunology and infection. She then obtained an MSc in neuroimmunology from McGill University. Following this, Aja returned to the University of Alberta for her PhD studies in comparative immunology, researching the role of macrophages in initiating and resolving inflammation in goldfish. She then moved to University of California-Berkeley for her postdoctoral fellowship in neuroimmunology. In her current role as the flow core manager at the University of Alberta, Faculty of Medicine and Dentistry, Aja oversees the operations of both the Flow Cytometry Facility and the High Content Analysis Core. She manages a team of cytometry technologists, with a specialty in imaging flow cytometry assay development. Aja is currently an ISAC SRL Emerging Leader.

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    Andrew Filby
    Director of the Newcastle University Cytometry and Single Cell Core Technology Unit
    Newcastle University

    Dr. Filby graduated summa cum laude from the University of Huddersfield with a first class honors in biochemistry. After graduating, he undertook a Ph.D. at the National Institute for Medical Research (NIMR) in Mill Hill, London. He worked on the Src family kinases LCK and Fyn in adaptive immunity, obtaining his PhD in molecular and cellular immunology from University College London (UCL).  Dr. Filby remained in the immunological field at the NIMR, working as a postdoctoral researcher on models of retroviral infection. He then worked in the commercial sector before taking up the deputy head role of the cytometry core at the London Research Institute (now the Francis Crick). Dr. Filby is currently director of the Newcastle University Cytometry and Single Cell Core Technology Unit. He leads a dedicated team of cytometry specialists with the sole aim of developing and implementing comprehensive, cutting-edge cytometry methods for the wider research community at Newcastle University and beyond. A significant part of his focus is the development of novel cytometry-based techniques that have underpinned several high-profile publications in journals including Science (2012, 2017, and 2018), Cell (2013), and Nature (2018). His current research is focused on whether label-free imaging cytometry techniques can be used to refine or replace the need for directed probes in order to prove cellular identity.

     Webinar Summary

    This webinar will give an overview of the current guidelines for publishing flow data with a high level of rigor. We will discuss publication of both standard flow cytometry data, as well as imaging cytometry, mass cytometry, and genomic cytometry data sets.

    Learning Objectives

    • Understand MIFlowCyt guidelines for publishing flow cytometry data.
    • Use best practices for communicating cytometry data in publications.
    • Review key points to include in any methods section toward reproducibility.
    • Explore data repositories.

    Who Should Attend

    Anyone interested in publishing high-quality, rigorous flow cytometry data.

    CMLE Credit: .75

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  • Contains 3 Component(s), Includes Credits Recorded On: 09/17/2019

    A CYTO U Webinar presented by Alex Skovsbo Jørgensen Keywords: Cell death, Annexin V, Necrosis, apoptosis, fixable viability dye, TUNEL assay, DNA damage, membrane integrity, DNA intercalating dye, membrane potential

    About the Presenter

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    Alex Skovsbo Jørgensen
    Assistant Professor
    Department of Health Science and Technology
    Aalborg University

    Alex is an assistant professor in the Department of Health Science and Technology at Aalborg University. His research focus is using machine learning and image analysis within the domain of digital pathology. His current research topics of interest within digital pathology is automated cancer detection and grading, artificial intelligence, and quality assessment of staining protocols. 

    Webinar Summary

    Breast cancer is the most frequent cancer among women worldwide. Ki67 can be used as an immunohistochemical pseudo marker for cell proliferation to determine how aggressive the cancer is and thereby the treatment of the patient. No standard Ki67 staining protocol exists, resulting in interlaboratory stain variability. Therefore, it is important to determine the quality control of a staining protocol to ensure correct diagnosis and treatment of patients. Currently, quality control is performed by the organization NordiQC that use an expert panel-based qualitative assessment system. However, no objective method exists to determine the quality of a staining protocol.

    Learning Objectives

    •     Understand the challenges of staining quality assessment.
    •     Use cell lines for assessment of stain quality.
    •     How to use image analysis and machine learning for quality assessment of staining protocols.
    •     Understand validation challenges.

    Who Should Attend

    Pathologists and engineers within medial image analysis and machine learning.

    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits Recorded On: 08/15/2019

    A CYTO U Webinar presented by Dagna Sheerar, SCYM Keywords: Reproducability, Statistical power, FMO, MIFlowCyt, Stain index

    About the Presenter

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    Dagna Sheerar, SCYM
    Manager
    University of Wisconsin Carbone Cancer Center (UWCCC) Flow Lab

    Dagna Sheerar has been working in flow cytometry shared resource laboratories since 2000, starting out as an assistant in organizing a BSL-3 cell sorting facility at the Immunology Services Laboratory of the Wisconsin National Primate Research Center. From there, she went on to manage the Flow Lab at the University of Western Ontario for three years. In 2006, Ms. Sheerar returned to the University of Wisconsin—Madison to work in the Carbone Comprehensive Cancer Center’s Flow Cytometry Laboratory. In 2011, she was hired as the manager of the UWCCC Flow Lab. Always an active member in the flow cytometry community, Ms. Sheerar is a member of the Steering Committee for the Great Lakes International Imaging and Flow Cytometry Association and a member of the ISAC Shared Resources Laboratory Educational Task Force. As manager of the UWCCC Flow Lab, Ms. Sheerar focuses on providing researchers with the tools and support to perform rigorous and reproducible flow cytometry assays in basic research and clinical research trials.

    Webinar Summary

    This webinar will outline the steps and considerations in designing a successful flow cytometry assay in the context of basic research and clinical research trials. We will focus on how best to minimize variables for a robust and reproducible assay, paying attention to producing data sets well suited to downstream computational data analysis platforms.

    Learning Objectives

    • Discuss the importance of working with biostatisticians in the early experimental planning stages.
    • Describe how to create criteria for sample inclusion/exclusion and building in room for sample loss.
    • Learn the importance of validating reagents and proper quality control and characterization of instrumentation.
    • Discuss how to create rigorous protocols and the importance of record keeping and annotation.
    • Expectations for the design, optimization, and standardization of assays and data analysis pipelines.

    Who Should Attend

    • Researchers using flow cytometry assays in the course of their research.
    • SRL staff supporting researchers in the design, optimization, standardization, and data analysis of these research projects.
    • Computational biologists and biostatisticians performing data analyses for large-scale flow cytometry based experiments.

    CMLE Credit: 1.0

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  • Contains 4 Product(s)

    Package for cell sorting in cytometry.

    • Cell Sorting: Fundamentals and Selected Novel Applications
    • Cell Sorting for Function and Viability
    • 3D Cytometry: Intelligent Image—Activated Cell Sorting
    • Current Standards in Flow Cytometry Cell Sorter Biosafety
    • Intelligent Image-Activated Cell Sorting: A Tutorial 
    • Optimizing SRL Performance: Boost your Cell Sorting Capacity
  • Contains 3 Product(s)

    A three-part CYTO U Webinar series on Validation, the Key to Translatable Flow Cytometry Keywords: Longitudinal, IQ, OQ, PQ, ERF, Inter-instrument

    Instrument qualification and method validation are two of the pillars required for obtaining cytometry data that are reliable and suitable for decision making. In this three-part webinar series all aspects of validation for flow cytometry, from the instrument to the assay, will be covered.  

    Validation, the Key to Translatable Flow Cytometry, Part 1: Method Validation—Overview, Concepts

    Validation, the Key to Translatable Flow Cytometry, Part 2: Method Validation—Planning and Executing

    Validation, the Key to Translatable Flow Cytometry, Part 3: Instrument Qualification

  • Contains 4 Component(s), Includes Credits Recorded On: 10/15/2018

    A CYTO U Webinar presented by Jennifer Wilshire, PhD & Tomas Baumgartner Keywords: panel design, spillover spreading, spillover spreading matrix, resolution impact matrix, sentinel panel design, brightness, compensation, controls, spectra

    The Presenters

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    Jennifer Wilshire, PhD
    Assistant Manager
    Memorial Sloan Kettering Cancer Center

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    Tomas Baumgartner
    Manager
    Flow Cytometry Core Facility
    Weill Cornell Medicine

    Webinar Description

    This webinar will focus on the process of building multicolor panels. We will break the process down into step-by-step guides based on the number of colors in your panel. This webinar will also provide a framework for those teaching multicolor panel design in an educational setting such as a Shared Resource Lab.

    Learning Objectives

    • Know the three processes used to build multicolor panels.
    • Choose the correct step-by-step process based on the number of colors in the panel.
    • Interpret spillover spreading matrix/resolution impact matrix to determine which colors contribute spreading to others.
    • Practice building a multicolor panel using all the tricks and tips discussed in the webinar.

    Who Should Attend

    Anyone who is designing multicolor panels or teaching others how to design multiparameter panels.

    CMLE Credit: 1.0

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  • Contains 4 Component(s), Includes Credits Recorded On: 04/04/2018

    A CYTO U Webinar presented by Joanne Lannigan, MS Keywords: imaging flow cytometry, microscopy, imagestream, imaging

    About the Presenter

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    Joanne Lannigan, MS
    Director of the Flow Cytometry Core Facility 
    School of Medicine at the University of Virginia

    Joanne Lannigan was an early adopter of Imaging Flow Cytometry (IFC). Having received the fifth commercially produced IS100 instrument in 2005, she has worked with many investigators to develop numerous protocols and applications for this technology. Her work, and the work of her colleagues, has appeared in many different journals, including journal front covers, and has also been published in Current Protocols in Cytometry. Her lab has continued to upgrade IFC instrumentation over the years and currently uses a four laser, multi-magnification two camera, Imagestream MKII system to explore ways to utilize this technology to answer scientific questions not well served by conventional flow cytometry or traditional fluorescence microscopy. Her most recent interests involve the use of IFC for the analysis of submicron particles (extracellular vesicles, viruses, and other nanoparticles).  

    Webinar Summary

    Conventional flow cytometry has been an extremely valuable tool due to its high-parameter and high-throughput capabilities for single cell analyses. Using fluorescence and scatter measurements, it has the ability to analyze many different cellular components on thousands to millions of cells in relatively short acquisition times, providing an efficient technology for robust statistical analyses. The limitations of conventional flow cytometry, however, are the lack of morphological information and fluorescence localization, such as is possible with fluorescence microscopy. Fluorescence microscopy on the other hand, while providing morphological and fluorescence localization information, lacks the high throughput and hence the statistical power of flow cytometry. In addition, there is an inherent bias in the image field selection for analysis.

    Learning Objectives

    Ideally, a technology which can provide unbiased high-parameter, high-throughput flow cytometric data, while also providing morphological and fluorescence localization information would be a very valuable tool. Imaging Flow Cytometry (IFC) as provided by the Imagestream MKII and Flowsight instruments are such tools. In this webinar, you will learn how this technology works, as well as how this technology has enabled studies which typically would be very difficult to achieve by conventional flow cytometry. A number of published applications will be presented.

    CMLE Credit: 1.0

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  • Contains 7 Product(s)

    This 7-part webinar series explores the best practices of a successful SRL facility, including data management, SOPs, training & education, safety, QA, operations, and staffing.

    Part 1: Managing Data in a Flow Cytometry Core

    Part 2: Standard Operating Procedures

    Part 3: Training and Education

    Part 4: Laboratory Safety

    Part 5: Quality Control / Quality Assurance

    Part 6: Operations

    Part 7: Staffing

  • Contains 4 Component(s), Includes Credits Recorded On: 04/25/2017

    A CYTO U Webinar presented by Winfried Wiegraebe, PhD Keywords: Quantitative microscopy, Cell Biology, Image analysis, human stem cells, virtual cell

    About the Presenter

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    Winfried Wiegraebe, PhD
    Director for Microscopy and Image Analysis
    Allen Institute for Cell Science

    Winfried Wiegraebe received his diploma in biophysics and machine tools and industrial management from the Technical University in Munich, Germany. For his diploma and PhD thesis he joined the Department of Molecular Structural Biology headed by Wolfgang Baumeister at the Max-Planck Institute for Biochemistry in Martinsried, Germany. Under the mentorship of Reinhard "Guckus" Guckenberger, Winfried investigated hydrated bacterial surface proteins with scanning tunneling microscopy (STM). He developed an atomic force microscope (AFM) to measure their conductivity. He complemented this data with measurements of their local elasticity and friction. In 2001, Winfried joined the Carl Zeiss group in the USA and helped customers to be successful users of confocal and two-photon microscopes (NLO) as well as FCS.

    In 2005 Winfried moved back to academia and joined the Stowers Institute for Medical Research in Kansas City, Missouri, USA. He created the group for Advanced Instrumentation and Physics and later became the head of the Stowers Microscopy Center. In this capacity he supported and built with his team a large variety of microscope techniques, from laser micro-dissection to super-resolution techniques and light sheet microscopy. He developed technology to automatically perform FCS measurements on 4000 different proteins in yeast.

    Webinar Summary 

    At the Allen Institute for Cell Science, we believe that understanding the organization of healthy, living cells and their changes during growth, differentiation, and other processes is an essential starting point to understanding cellular changes caused by disease. The textbook cartoons of the human cells that we are familiar with are based more on imagination than on solid data. To develop the needed image-based cellular data, we are developing a pipeline to create large, high replicate data sets for us and other scientists in academia and industries to analyze, model, and generate new hypotheses about cellular behaviors. We use gene-edited hiPS cells, since they are diploid, relatively homogeneous, and can be induced to differentiate into many other cell types. In the first iteration, we are identifying the locations of the major cellular machines and signaling pathways using genome-edited, fluorescently tagged proteins that we image with automated light microscopes. We are analyzing these image-data using statistical models.

    CMLE Credit: 1.0